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Cytoskeleton (Hoboken)
2012 Nov 01;6911:973-82. doi: 10.1002/cm.21068.
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Localization of cytokinesis factors to the future cell division site by microtubule-dependent transport.
Atilgan E
,
Burgess D
,
Chang F
.
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The mechanism by which spindle microtubules (MTs) determine the site of cell division in animal cells is still highly controversial. Putative cytokinesis "signals" have been proposed to be positioned by spindle MTs at equatorial cortical regions to increase cortical contractility and/or at polar regions to decrease contractility [Rappaport, 1986; von Dassow, 2009]. Given the relative paucity of MTs at the future division site, it has not been clear how MTs localize cytokinesis factors there. Here, we test cytokinesis models using computational and experimental approaches. We present a simple lattice-based model in which signal-kinesin complexes move by transient plus-end directed movements on MTs interspersed with occasions of uniform diffusion in the cytoplasm. In simulations, complexes distribute themselves initially at the spindle midzone and then move on astral MTs to accumulate with time at the equatorial cortex. Simulations accurately predict cleavage patterns of cells with different geometries and MT arrangements and elucidate several experimental observations that have defied easy explanation by previous models. We verify this model with experiments on indented sea urchin zygotes showing that cells often divide perpendicular to the spindle at sites distinct from the indentations. These studies support an equatorial stimulation model and provide a simple mechanism explaining how cytokinesis factors localize to the future division site.
Alsop,
Microtubules are the only structural constituent of the spindle apparatus required for induction of cell cleavage.
2003, Pubmed
Alsop,
Microtubules are the only structural constituent of the spindle apparatus required for induction of cell cleavage.
2003,
Pubmed
Asnes,
Cell cleavage. Ultrastructural evidence against equatorial stimulation by aster microtubules.
1979,
Pubmed
,
Echinobase
Baruni,
Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C. elegans embryos.
2008,
Pubmed
,
Echinobase
Bement,
A microtubule-dependent zone of active RhoA during cleavage plane specification.
2005,
Pubmed
,
Echinobase
Bonaccorsi,
Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster.
1998,
Pubmed
Bringmann,
A cytokinesis furrow is positioned by two consecutive signals.
2005,
Pubmed
Burgess,
Site selection for the cleavage furrow at cytokinesis.
2005,
Pubmed
Canman,
Determining the position of the cell division plane.
2003,
Pubmed
Cao,
Mechanism of the formation of contractile ring in dividing cultured animal cells. II. Cortical movement of microinjected actin filaments.
1990,
Pubmed
Dechant,
Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation.
2003,
Pubmed
Devore,
A model for astral stimulation of cytokinesis in animal cells.
1989,
Pubmed
Earnshaw,
Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation.
1991,
Pubmed
Fishkind,
Orientation and three-dimensional organization of actin filaments in dividing cultured cells.
1993,
Pubmed
Foe,
Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation.
2008,
Pubmed
,
Echinobase
Hu,
Cell polarization during monopolar cytokinesis.
2008,
Pubmed
Hutterer,
Clustering of centralspindlin is essential for its accumulation to the central spindle and the midbody.
2009,
Pubmed
Inoue,
Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression.
2004,
Pubmed
KAWAMURA,
Studies on cytokinesis in neuroblasts of the grasshopper, Chortophaga viridifasciata (De Geer). I. Formation and behavior of the mitotic apparatus.
1960,
Pubmed
Khodjakov,
Centrosome-independent mitotic spindle formation in vertebrates.
2000,
Pubmed
Klumpp,
Self-organized density patterns of molecular motors in arrays of cytoskeletal filaments.
2005,
Pubmed
Lewellyn,
The chromosomal passenger complex and centralspindlin independently contribute to contractile ring assembly.
2011,
Pubmed
Megraw,
Zygotic development without functional mitotic centrosomes.
2001,
Pubmed
Minc,
Influence of cell geometry on division-plane positioning.
2011,
Pubmed
,
Echinobase
Mishima,
Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.
2002,
Pubmed
Motegi,
Two phases of astral microtubule activity during cytokinesis in C. elegans embryos.
2006,
Pubmed
Murata-Hori,
Both midzone and astral microtubules are involved in the delivery of cytokinesis signals: insights from the mobility of aurora B.
2002,
Pubmed
Ng,
Movement of membrane domains and requirement of membrane signaling molecules for cytokinesis.
2005,
Pubmed
,
Echinobase
Nédélec,
Dynamic concentration of motors in microtubule arrays.
2001,
Pubmed
Odell,
An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning.
2008,
Pubmed
,
Echinobase
RAPPAPORT,
Experiments concerning the cleavage stimulus in sand dollar eggs.
1961,
Pubmed
,
Echinobase
Rappaport,
Division of constricted and urethane-treated sand dollar eggs: a test of the polar stimulation hypothesis.
1984,
Pubmed
,
Echinobase
Rappaport,
Repeated furrow formation from a single mitotic apparatus in cylindrical sand dollar eggs.
1985,
Pubmed
,
Echinobase
Rappaport,
Establishment of the mechanism of cytokinesis in animal cells.
1986,
Pubmed
Rappaport,
Cleavage in conical sand dollar eggs.
1994,
Pubmed
,
Echinobase
Rieder,
Mitosis in vertebrate somatic cells with two spindles: implications for the metaphase/anaphase transition checkpoint and cleavage.
1997,
Pubmed
Roberts-Galbraith,
Setting the F-BAR: functions and regulation of the F-BAR protein family.
2010,
Pubmed
Rogers,
Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner.
2004,
Pubmed
Sanger,
Unusual cleavage furrows in vertebrate tissue culture cells: insights into the mechanisms of cytokinesis.
1998,
Pubmed
Shlomovitz,
Physical model of contractile ring initiation in dividing cells.
2008,
Pubmed
Shuster,
Transitions regulating the timing of cytokinesis in embryonic cells.
2002,
Pubmed
,
Echinobase
Siller,
Spindle orientation during asymmetric cell division.
2009,
Pubmed
Strickland,
Induction of cytokinesis is independent of precisely regulated microtubule dynamics.
2005,
Pubmed
,
Echinobase
Strickland,
Interaction between EB1 and p150glued is required for anaphase astral microtubule elongation and stimulation of cytokinesis.
2005,
Pubmed
,
Echinobase
Vagnarelli,
Chromosomal passengers: the four-dimensional regulation of mitotic events.
2004,
Pubmed
Wang,
The mechanism of cytokinesis: reconsideration and reconciliation.
2001,
Pubmed
Werner,
Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis.
2007,
Pubmed
White,
On the mechanisms of cytokinesis in animal cells.
1983,
Pubmed
Wühr,
A model for cleavage plane determination in early amphibian and fish embryos.
2010,
Pubmed
von Dassow,
Concurrent cues for cytokinetic furrow induction in animal cells.
2009,
Pubmed
von Dassow,
Action at a distance during cytokinesis.
2009,
Pubmed
,
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