Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mar Biotechnol (NY)
2013 Feb 01;151:73-86. doi: 10.1007/s10126-012-9460-5.
Show Gene links
Show Anatomy links
Mannan-binding lectin of the sea urchin Strongylocentrotus nudus.
Bulgakov AA
,
Eliseikina MG
,
Kovalchuk SN
,
Petrova IY
,
Likhatskaya GN
,
Shamshurina EV
,
Rasskazov VA
.
???displayArticle.abstract???
A novel lectin specific to low-branched mannans (MBL-SN) was isolated from coelomic plasma of the sea urchin Strongylocentrotus nudus by combining anion-exchange liquid chromatography on DEAE Toyopearl 650 M, affinity chromatography on mannan-Sepharose and gel filtration on the Sephacryl S-200. The molecular mass of MBL-SN was estimated by sodium dodecyl sulphate polyacrylamide gel electrophoresis under non-reducing conditions to be about 34 kDa. MBL-SN was shown to be a dimer with two identical subunits of about 17 kDa. The native MBL-SN exists as a tetramer. The physico-chemical properties of MBL-SN indicate that it belongs to C-type mannan-binding lectins. The cDNA encoding MBL-SN was cloned from the total cDNA of S. nudus coelomocytes and encodes a 17-kDa protein of 144 amino acid residues that contains a single carbohydrate-recognition domain of C-type lectins. Prediction of the MBL-SN tertiary structure using comparative modelling revealed that MBL-SN is an α/β-protein with eight β-strands and two α-helices. Comparison of the MBL-SN model with available three-dimensional structures of C-type lectins revealed that they share a common fold pattern.
Anders,
Complement-dependent neutralization of influenza virus by a serum mannose-binding lectin.
1994, Pubmed
Anders,
Complement-dependent neutralization of influenza virus by a serum mannose-binding lectin.
1994,
Pubmed
Arnold,
The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling.
2006,
Pubmed
Blair,
Molecular phylogeny and divergence times of deuterostome animals.
2005,
Pubmed
Bottjer,
Paleogenomics of echinoderms.
2006,
Pubmed
,
Echinobase
Bulgakov,
Isolation and properties of a mannan-binding lectin from the coelomic fluid of the holothurian Cucumaria japonica.
2000,
Pubmed
,
Echinobase
Bulgakov,
Molecular and biological characterization of a mannan-binding lectin from the holothurian Apostichopus japonicus.
2007,
Pubmed
,
Echinobase
Chen,
Detection and characterization of a mannan-binding lectin from the mosquito, Anopheles stephensi (Liston).
1999,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Drickamer,
Biology of animal lectins.
1993,
Pubmed
Feinberg,
Structural basis for langerin recognition of diverse pathogen and mammalian glycans through a single binding site.
2011,
Pubmed
GRABAR,
[Method permitting the combined study of the electrophoretic and the immunochemical properties of protein mixtures; application to blood serum].
1953,
Pubmed
Gadjeva,
Mannan-binding lectin--a soluble pattern recognition molecule.
2004,
Pubmed
Gourdine,
The three-dimensional structure of codakine and related marine C-type lectins.
2007,
Pubmed
Guex,
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling.
1997,
Pubmed
Guo,
Structural basis for distinct ligand-binding and targeting properties of the receptors DC-SIGN and DC-SIGNR.
2004,
Pubmed
Hibino,
The immune gene repertoire encoded in the purple sea urchin genome.
2006,
Pubmed
,
Echinobase
Hinman,
Developmental gene regulatory network architecture across 500 million years of echinoderm evolution.
2003,
Pubmed
,
Echinobase
Holmskov,
Collectins: collagenous C-type lectins of the innate immune defense system.
1994,
Pubmed
Holmskov,
Collections and ficolins: humoral lectins of the innate immune defense.
2003,
Pubmed
Ikeda,
Serum lectin with known structure activates complement through the classical pathway.
1987,
Pubmed
Kawasaki,
Structure and biology of mannan-binding protein, MBP, an important component of innate immunity.
1999,
Pubmed
Kelley,
Protein structure prediction on the Web: a case study using the Phyre server.
2009,
Pubmed
Kiefer,
The SWISS-MODEL Repository and associated resources.
2009,
Pubmed
Kurata,
Structure and function of mannan-binding proteins isolated from human liver and serum.
1994,
Pubmed
Kvennefors,
An ancient and variable mannose-binding lectin from the coral Acropora millepora binds both pathogens and symbionts.
2008,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Matz,
Amplification of cDNA ends based on template-switching effect and step-out PCR.
1999,
Pubmed
Medzhitov,
Recognition of microorganisms and activation of the immune response.
2007,
Pubmed
Mogues,
Characterization of two mannose-binding protein cDNAs from rhesus monkey (Macaca mulatta): structure and evolutionary implications.
1996,
Pubmed
Muramoto,
The amino-acid sequence of multiple lectins of the acorn barnacle Megabalanus rosa and its homology with animal lectins.
1990,
Pubmed
,
Echinobase
Nakane,
Peroxidase-labeled antibody. A new method of conjugation.
1974,
Pubmed
Nikolakopoulou,
Molecular cloning and characterisation of two homologues of Mannose-Binding Lectin in rainbow trout.
2006,
Pubmed
Petersen,
The mannan-binding lectin pathway of complement activation: biology and disease association.
2001,
Pubmed
Sato,
Molecular characterization of a novel serine protease involved in activation of the complement system by mannose-binding protein.
1994,
Pubmed
Schultz,
SMART: a web-based tool for the study of genetically mobile domains.
2000,
Pubmed
Shirai,
Structure of rhamnose-binding lectin CSL3: unique pseudo-tetrameric architecture of a pattern recognition protein.
2009,
Pubmed
Storgaard,
Isolation and characterization of porcine mannan-binding proteins of different size and ultrastructure.
1996,
Pubmed
Takahashi,
Cloning and characterization of mannose-binding lectin from lamprey (Agnathans).
2006,
Pubmed
Teillet,
The two major oligomeric forms of human mannan-binding lectin: chemical characterization, carbohydrate-binding properties, and interaction with MBL-associated serine proteases.
2005,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Turner,
The role of mannose-binding lectin in health and disease.
2003,
Pubmed
Turner,
Mannose-binding lectin: the pluripotent molecule of the innate immune system.
1996,
Pubmed
Weis,
The C-type lectin superfamily in the immune system.
1998,
Pubmed
Weis,
Structure of a C-type mannose-binding protein complexed with an oligosaccharide.
1992,
Pubmed
Weis,
Trimeric structure of a C-type mannose-binding protein.
1994,
Pubmed
Westbrook,
The Protein Data Bank and structural genomics.
2003,
Pubmed
Wiens,
Molecular control of serial module formation along the apical-basal axis in the sponge Lubomirskia baicalensis: silicateins, mannose-binding lectin and mago nashi.
2006,
Pubmed
Zelensky,
Comparative analysis of structural properties of the C-type-lectin-like domain (CTLD).
2003,
Pubmed
