ECB-ART-42372
Proc Natl Acad Sci U S A
2012 Apr 03;10914:5334-9. doi: 10.1073/pnas.1117846109.
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Pantropic retroviruses as a transduction tool for sea urchin embryos.
Core AB
,
Reyna AE
,
Conaway EA
,
Bradham CA
.
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Sea urchins are an important model for experiments at the intersection of development and systems biology, and technical innovations that enhance the utility of this model are of great value. This study explores pantropic retroviruses as a transduction tool for sea urchin embryos, and demonstrates that pantropic retroviruses infect sea urchin embryos with high efficiency and genomically integrate at a copy number of one per cell. We successfully used a self-inactivation strategy to both insert a sea urchin-specific enhancer and disrupt the endogenous viral enhancer. The resulting self-inactivating viruses drive global and persistent gene expression, consistent with genomic integration during the first cell cycle. Together, these data provide substantial proof of principle for transduction technology in sea urchin embryos.
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Genes referenced: LOC100887844 LOC115919910
References [+] :
Akasaka,
Upstream element of the sea urchin arylsulfatase gene serves as an insulator.
1999, Pubmed,
Echinobase
Akasaka, Upstream element of the sea urchin arylsulfatase gene serves as an insulator. 1999, Pubmed , Echinobase
Arnone, Green Fluorescent Protein in the sea urchin: new experimental approaches to transcriptional regulatory analysis in embryos and larvae. 1997, Pubmed , Echinobase
Barzon, Transcriptionally targeted retroviral vector for combined suicide and immunomodulating gene therapy of thyroid cancer. 2002, Pubmed
Baum, Novel retroviral vectors for efficient expression of the multidrug resistance (mdr-1) gene in early hematopoietic cells. 1995, Pubmed
Bigornia, Construction and in vitro characterization of attenuated feline immunodeficiency virus long terminal repeat mutant viruses. 2001, Pubmed
Boulo, Infection of cultured embryo cells of the pacific oyster, Crassostrea gigas, by pantropic retroviral vectors. 2000, Pubmed
Bradham, Chordin is required for neural but not axial development in sea urchin embryos. 2009, Pubmed , Echinobase
Bradham, p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos. 2006, Pubmed , Echinobase
Brown, New computational approaches for analysis of cis-regulatory networks. 2002, Pubmed , Echinobase
Burns, Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. 1993, Pubmed
Burns, Pantropic retroviral vector-mediated gene transfer, integration, and expression in cultured newt limb cells. 1994, Pubmed
Burns, Retrovirol gene transfer in Xenopus cell lines and embryos. 1996, Pubmed
Chun, In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency. 1995, Pubmed
Costantino, A preformed compact ribosome-binding domain in the cricket paralysis-like virus IRES RNAs. 2005, Pubmed
Couture, Retroviral vectors containing chimeric promoter/enhancer elements exhibit cell-type-specific gene expression. 1994, Pubmed
Davidson, A genomic regulatory network for development. 2002, Pubmed , Echinobase
Davidson, A provisional regulatory gene network for specification of endomesoderm in the sea urchin embryo. 2002, Pubmed , Echinobase
Deniz, Translation initiation factors are not required for Dicistroviridae IRES function in vivo. 2009, Pubmed
Emerman, Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism. 1984, Pubmed
Epel, Methods for removal of the vitelline membrane of sea urchin eggs. II. Controlled exposure to trypsin to eliminate post-fertilization clumping of embryos. 1970, Pubmed , Echinobase
Ettensohn, Lessons from a gene regulatory network: echinoderm skeletogenesis provides insights into evolution, plasticity and morphogenesis. 2009, Pubmed , Echinobase
Flytzanis, Persistence and integration of cloned DNA in postembryonic sea urchins. 1985, Pubmed , Echinobase
Gan, An orthodenticle-related protein from Strongylocentrotus purpuratus. 1995, Pubmed , Echinobase
Goff, Operating under a Gag order: a block against incoming virus by the Fv1 gene. 1996, Pubmed
Hinman, Developmental gene regulatory network architecture across 500 million years of echinoderm evolution. 2003, Pubmed , Echinobase
Hough-Evans, Mosaic incorporation and regulated expression of an exogenous gene in the sea urchin embryo. 1988, Pubmed , Echinobase
Hu, A novel immortalization vector for the establishment of penaeid shrimp cell lines. 2008, Pubmed
Indraccolo, Expression from cell type-specific enhancer-modified retroviral vectors after transduction: influence of marker gene stability. 2002, Pubmed
Jan, Factorless ribosome assembly on the internal ribosome entry site of cricket paralysis virus. 2002, Pubmed
Jähner, De novo methylation and expression of retroviral genomes during mouse embryogenesis. 1982, Pubmed
Kines, Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs. 2010, Pubmed
Kirchhamer, Spatial and temporal information processing in the sea urchin embryo: modular and intramodular organization of the CyIIIa gene cis-regulatory system. 1996, Pubmed , Echinobase
Li, Requirement of SpOtx in cell fate decisions in the sea urchin embryo and possible role as a mediator of beta-catenin signaling. 1999, Pubmed , Echinobase
Lin, Integration and germ-line transmission of a pseudotyped retroviral vector in zebrafish. 1994, Pubmed
Lu, Production of transgenic dwarf surfclams, Mulinia lateralis, with pantropic retroviral vectors. 1996, Pubmed
Materna, High accuracy, high-resolution prevalence measurement for the majority of locally expressed regulatory genes in early sea urchin development. 2010, Pubmed , Echinobase
Matsubara, Pantropic retroviral vectors integrate and express in cells of the malaria mosquito, Anopheles gambiae. 1996, Pubmed
McGee-Estrada, A Moloney murine leukemia virus driven by the Jaagsiekte sheep retrovirus enhancers shows enhanced specificity for infectivity in lung epithelial cells. 2005, Pubmed
Miller, Retroviral vectors. 1992, Pubmed
Minokawa, Expression patterns of four different regulatory genes that function during sea urchin development. 2004, Pubmed , Echinobase
Nam, Functional cis-regulatory genomics for systems biology. 2010, Pubmed , Echinobase
Nam, Cis-regulatory control of the nodal gene, initiator of the sea urchin oral ectoderm gene network. 2007, Pubmed , Echinobase
Nethe, Retroviral superinfection resistance. 2005, Pubmed
Oliveri, Global regulatory logic for specification of an embryonic cell lineage. 2008, Pubmed , Echinobase
Pannell, Retrovirus vector silencing is de novo methylase independent and marked by a repressive histone code. 2000, Pubmed
Pestova, Translation elongation after assembly of ribosomes on the Cricket paralysis virus internal ribosomal entry site without initiation factors or initiator tRNA. 2003, Pubmed
Pestova, Position of the CrPV IRES on the 40S subunit and factor dependence of IRES/80S ribosome assembly. 2004, Pubmed
Peter, Evolution of gene regulatory networks controlling body plan development. 2011, Pubmed
Peter, A gene regulatory network controlling the embryonic specification of endoderm. 2011, Pubmed , Echinobase
Pryciak, Fv-1 restriction and its effects on murine leukemia virus integration in vivo and in vitro. 1992, Pubmed
Que, Pantropic retroviral vectors mediate gene transfer and expression in Entamoeba histolytica. 1999, Pubmed
Range, Cis-regulatory analysis of nodal and maternal control of dorsal-ventral axis formation by Univin, a TGF-beta related to Vg1. 2007, Pubmed , Echinobase
Rast, brachyury Target genes in the early sea urchin embryo isolated by differential macroarray screening. 2002, Pubmed , Echinobase
Salmons, Targeting of retroviral vectors for gene therapy. 1993, Pubmed
Samanta, The transcriptome of the sea urchin embryo. 2006, Pubmed , Echinobase
Saudemont, Ancestral regulatory circuits governing ectoderm patterning downstream of Nodal and BMP2/4 revealed by gene regulatory network analysis in an echinoderm. 2010, Pubmed , Echinobase
Sharma, Activation of the skeletogenic gene regulatory network in the early sea urchin embryo. 2010, Pubmed , Echinobase
Smith, A gene regulatory network subcircuit drives a dynamic pattern of gene expression. 2007, Pubmed , Echinobase
Sodergren, The genome of the sea urchin Strongylocentrotus purpuratus. 2006, Pubmed , Echinobase
Stewart, De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells. 1982, Pubmed
Su, A perturbation model of the gene regulatory network for oral and aboral ectoderm specification in the sea urchin embryo. 2009, Pubmed , Echinobase
Teich, Virus infection of murine teratocarcinoma stem cell lines. 1977, Pubmed
Titeux, SIN retroviral vectors expressing COL7A1 under human promoters for ex vivo gene therapy of recessive dystrophic epidermolysis bullosa. 2010, Pubmed
Vile, A comparison of the properties of different retroviral vectors containing the murine tyrosinase promoter to achieve transcriptionally targeted expression of the HSVtk or IL-2 genes. 1994, Pubmed
Vile, Tissue-specific gene expression from Mo-MLV retroviral vectors with hybrid LTRs containing the murine tyrosinase enhancer/promoter. 1995, Pubmed
Vile, Retroviruses as vectors. 1995, Pubmed
Wei, The sea urchin animal pole domain is a Six3-dependent neurogenic patterning center. 2009, Pubmed , Echinobase
Wei, A database of mRNA expression patterns for the sea urchin embryo. 2006, Pubmed , Echinobase
Whitcomb, Retroviral reverse transcription and integration: progress and problems. 1992, Pubmed
Yaguchi, A Wnt-FoxQ2-nodal pathway links primary and secondary axis specification in sea urchin embryos. 2008, Pubmed , Echinobase
Yuh, An otx cis-regulatory module: a key node in the sea urchin endomesoderm gene regulatory network. 2004, Pubmed , Echinobase
Yuh, Cis-regulatory logic in the endo16 gene: switching from a specification to a differentiation mode of control. 2001, Pubmed , Echinobase
Yuh, Patchy interspecific sequence similarities efficiently identify positive cis-regulatory elements in the sea urchin. 2002, Pubmed , Echinobase
Yuh, Genomic cis-regulatory logic: experimental and computational analysis of a sea urchin gene. 1998, Pubmed , Echinobase
Zhu, A large-scale analysis of mRNAs expressed by primary mesenchyme cells of the sea urchin embryo. 2001, Pubmed , Echinobase
de Breyne, Direct functional interaction of initiation factor eIF4G with type 1 internal ribosomal entry sites. 2009, Pubmed
de-Leon, Information processing at the foxa node of the sea urchin endomesoderm specification network. 2010, Pubmed , Echinobase