ECB-ART-41786
Acta Biochim Biophys Sin (Shanghai)
2010 Nov 01;4211:779-86. doi: 10.1093/abbs/gmq092.
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Zinc induces unfolding and aggregation of dimeric arginine kinase by trapping reversible unfolding intermediate.
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Arginine kinase plays an important role in the cellular energy metabolism of invertebrates. Dimeric arginine kinase (dAK) is unique in some marine invertebrates. The effects of Zn²(+) on the unfolding and aggregation of dAK from the sea cucumber Stichopus japonicus were investigated. Our results indicated that Zn²(+) caused dAK inactivation accompanied by conformational unfolding, the exposure of hydrophobic surface, and aggregation. Kinetic studies showed the inactivation and unfolding of dAK followed biphasic kinetic courses. Zn²(+) can affect unfolding and refolding of dAK by trapping the reversible intermediate. Our study provides important information regarding the effect of Zn²(+) on metabolic enzymes in marine invertebrates.
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Genes referenced: LOC100887844 LOC105440390