ECB-ART-41382Dev Biol 2010 Apr 15;3402:188-99. doi: 10.1016/j.ydbio.2009.10.037.
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The endoderm gene regulatory network in sea urchin embryos up to mid-blastula stage.
As the result of early specification processes, sea urchin embryos eventually form various mesodermal cell lineages and a gut consisting of fore-, mid- and hindgut. The progression of specification as well as the overall spatial organization of the organism is encoded in its gene regulatory networks (GRNs). We have analyzed the GRN driving endoderm specification up to the onset of gastrulation and present in this paper the mechanisms which determine this process up to mid-blastula stage. At this stage, the embryo consists of two separate lineages of endoderm precursor cells with distinct regulatory states. One of these lineages, the veg2 cell lineage, gives rise to endoderm and mesoderm cell types. The separation of these cell fates is initiated by the spatially confined activation of the mesoderm GRN superimposed on a generally activated endoderm GRN within veg2 descendants. Here we integrate the architecture of regulatory interactions with the spatial restriction of regulatory gene expression to model the logic control of endoderm development.
PubMed ID: 19895806
PMC ID: PMC3981691
Article link: Dev Biol
Genes referenced: LOC100887844 LOC115919910 LOC575170
Morpholinos: LOC373243 MO2 evxl MO1 foxa1 MO1 hbox7 MO2 myc MO1 otx2 MO2 otx2 MO3 prdm1 MO3 sox4 MO1 tbxt MO1
References [+] :
Arenas-Mena, Hindgut specification and cell-adhesion functions of Sphox11/13b in the endoderm of the sea urchin embryo. 2007, Pubmed, Echinobase