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Ecotoxicology
2010 Mar 01;193:555-62. doi: 10.1007/s10646-009-0432-0.
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Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects.
Pinsino A
,
Matranga V
,
Trinchella F
,
Roccheri MC
.
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In the marine environment increasing concentrations of bio-available compounds often result from anthropogenic activities. Among metal ions, manganese represents a new emergent factor in environmental contamination. Here, we studied the effects of manganese on Paracentrotus lividus sea urchin embryos using biological and biochemical approaches for the analysis of impact on development, tissue accumulation and stress markers. Embryos were continuously exposed from fertilization to manganese at concentrations ranging from 1.0 to 61.6 mg l(-1), monitored for developmental abnormalities at 48 h after fertilization, and used for atomic spectrometric analysis at various times from 6 to 72 h. We found that concentration- and time-dependent increases in morphological abnormalities were directly correlated to manganese accumulation, with major defects in skeleton formation at 48 h. Concurrently, we found an upregulation of the hsc70 and hsc60 stress proteins detected by immunoblotting, whereas no induction of apoptosis or ROS production was observed by TUNEL and live tests, respectively. Taken together, our findings demonstrate that the observed manganese embryo-toxicity is related to both its intracellular accumulation and misregulated homeostasis, and confirm the importance of stress proteins as protective agents in the acquisition of tolerance and resistance to apoptosis.
Baden,
Manganese accumulation by the antennule of the Norway lobster Nephrops norvegicus (L.) as a biomarker of hypoxic events.
2003, Pubmed
Baden,
Manganese accumulation by the antennule of the Norway lobster Nephrops norvegicus (L.) as a biomarker of hypoxic events.
2003,
Pubmed
Bellas,
Ecotoxicological evaluation of polycyclic aromatic hydrocarbons using marine invertebrate embryo-larval bioassays.
2008,
Pubmed
,
Echinobase
Bellas,
Integrative assessment of coastal pollution in a Ría coastal system (Galicia, NW Spain): correspondence between sediment chemistry and toxicity.
2008,
Pubmed
,
Echinobase
Bonaventura,
UVB radiation prevents skeleton growth and stimulates the expression of stress markers in sea urchin embryos.
2005,
Pubmed
,
Echinobase
Cakal Arslan,
Embryotoxic effects of nonylphenol and octylphenol in sea urchin Arbacia lixula.
2007,
Pubmed
,
Echinobase
Colomina,
Effect of day of exposure on the developmental toxicity of manganese in mice.
1996,
Pubmed
Daly,
A new perspective on radiation resistance based on Deinococcus radiodurans.
2009,
Pubmed
Diller,
Stress protein expression kinetics.
2006,
Pubmed
Frank,
Trophic cascades in a formerly cod-dominated ecosystem.
2005,
Pubmed
Geraci,
Nickel, lead, and cadmium induce differential cellular responses in sea urchin embryos by activating the synthesis of different HSP70s.
2004,
Pubmed
,
Echinobase
Gerber,
Carcinogenicity, mutagenicity and teratogenicity of manganese compounds.
2002,
Pubmed
Hamdoun,
Embryo stability and vulnerability in an always changing world.
2007,
Pubmed
Henry,
The development of dorsoventral and bilateral axial properties in sea urchin embryos.
1998,
Pubmed
,
Echinobase
Kobayashi,
Effects of heavy metals on sea urchin embryo development. Part 2. Interactive toxic effects of heavy metals in synthetic mine effluents.
2005,
Pubmed
,
Echinobase
Kültz,
Molecular and evolutionary basis of the cellular stress response.
2005,
Pubmed
Lima,
Genotoxic and cytotoxic effects of manganese chloride in cultured human lymphocytes treated in different phases of cell cycle.
2008,
Pubmed
Nahon,
Improved Comet assay for the assessment of UV genotoxicity in Mediterranean sea urchin eggs.
2008,
Pubmed
,
Echinobase
Nesatyy,
Analysis of environmental stress response on the proteome level.
2008,
Pubmed
Palotai,
Chaperones as integrators of cellular networks: changes of cellular integrity in stress and diseases.
2008,
Pubmed
Pinsino,
Sea urchin coelomocytes as a novel cellular biosensor of environmental stress: a field study in the Tremiti Island Marine Protected Area, Southern Adriatic Sea, Italy.
2008,
Pubmed
,
Echinobase
Radenac,
Bioaccumulation and toxicity of four dissolved metals in Paracentrotus lividus sea-urchin embryo.
2001,
Pubmed
,
Echinobase
Roccheri,
Cadmium induces the expression of specific stress proteins in sea urchin embryos.
2004,
Pubmed
,
Echinobase
Rosen,
Critical tissue copper residues for marine bivalve (Mytilus galloprovincialis) and echinoderm (Strongylocentrotus purpuratus) embryonic development: conceptual, regulatory and environmental implications.
2008,
Pubmed
,
Echinobase
Roth,
Homeostatic and toxic mechanisms regulating manganese uptake, retention, and elimination.
2006,
Pubmed
Russo,
Stress to cadmium monitored by metallothionein gene induction in Paracentrotus lividus embryos.
2003,
Pubmed
,
Echinobase
Santamaria,
Manganese exposure, essentiality & toxicity.
2008,
Pubmed
Satyanarayana,
Iron and manganese contamination: sources, adverse effects and control methods.
2007,
Pubmed
Torrente,
Effects of prenatal exposure to manganese on postnatal development and behavior in mice: influence of maternal restraint.
2002,
Pubmed
Van Baelen,
The Ca2+/Mn2+ pumps in the Golgi apparatus.
2004,
Pubmed
Weinstein,
Shell disease and metal content of blue crabs, Callinectes sapidus, from the Albemarle-Pamlico Estuarine System, North Carolina.
1992,
Pubmed
Zhang,
Changes in the brain mitochondrial proteome of male Sprague-Dawley rats treated with manganese chloride.
2005,
Pubmed
Zhang,
Effect of manganese on heat stress protein synthesis of new-born rats.
2002,
Pubmed
