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Dev Biol 2006 Dec 01;3001:194-218. doi: 10.1016/j.ydbio.2006.08.050.
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Protein tyrosine and serine-threonine phosphatases in the sea urchin, Strongylocentrotus purpuratus: identification and potential functions.

Byrum CA , Walton KD , Robertson AJ , Carbonneau S , Thomason RT , Coffman JA , McClay DR .

Protein phosphatases, in coordination with protein kinases, play crucial roles in regulation of signaling pathways. To identify protein tyrosine phosphatases (PTPs) and serine-threonine (ser-thr) phosphatases in the Strongylocentrotus purpuratus genome, 179 annotated sequences were studied (122 PTPs, 57 ser-thr phosphatases). Sequence analysis identified 91 phosphatases (33 conventional PTPs, 31 dual specificity phosphatases, 1 Class III Cysteine-based PTP, 1 Asp-based PTP, and 25 ser-thr phosphatases). Using catalytic sites, levels of conservation and constraint in amino acid sequence were examined. Nine of 25 receptor PTPs (RPTPs) corresponded to human, nematode, or fly homologues. Domain structure revealed that sea urchin-specific RPTPs including two, PTPRLec and PTPRscav, may act in immune defense. Embryonic transcription of each phosphatase was recorded from a high-density oligonucleotide tiling microarray experiment. Most RPTPs are expressed at very low levels, whereas nonreceptor PTPs (NRPTPs) are generally expressed at moderate levels. High expression was detected in MAP kinase phosphatases (MKPs) and numerous ser-thr phosphatases. For several expressed NRPTPs, MKPs, and ser-thr phosphatases, morpholino antisense-mediated knockdowns were performed and phenotypes obtained. Finally, to assess roles of annotated phosphatases in endomesoderm formation, a literature review of phosphatase functions in model organisms was superimposed on sea urchin developmental pathways to predict areas of functional activity.

PubMed ID: 17087928
PMC ID: PMC3045532
Article link: Dev Biol
Grant support: [+]

Genes referenced: LOC100887844 LOC115922275 LOC590297 thrb
Morpholinos: dusp1 MO1 dusp10 MO1 ppef1l MO1 ppm1b MO1 ppm1d MO1 ppm1g MO1 ppp1cal MO1 ppp1cal MO2 ppp1cb MO1 ppp2cb MO1 ppp4c MO1 ptpn1 MO1 ptpn9 MO1 tpa4a1 MO1

References [+] :
Alonso, Protein tyrosine phosphatases in the human genome. 2004, Pubmed