ECB-ART-39957
Dev Biol
2006 Dec 01;3001:219-37. doi: 10.1016/j.ydbio.2006.08.052.
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Analysis of cytoskeletal and motility proteins in the sea urchin genome assembly.
Morris RL
,
Hoffman MP
,
Obar RA
,
McCafferty SS
,
Gibbons IR
,
Leone AD
,
Cool J
,
Allgood EL
,
Musante AM
,
Judkins KM
,
Rossetti BJ
,
Rawson AP
,
Burgess DR
.
???displayArticle.abstract???
The sea urchin embryo is a classical model system for studying the role of the cytoskeleton in such events as fertilization, mitosis, cleavage, cell migration and gastrulation. We have conducted an analysis of gene models derived from the Strongylocentrotus purpuratus genome assembly and have gathered strong evidence for the existence of multiple gene families encoding cytoskeletal proteins and their regulators in sea urchin. While many cytoskeletal genes have been cloned from sea urchin with sequences already existing in public databases, genome analysis reveals a significantly higher degree of diversity within certain gene families. Furthermore, genes are described corresponding to homologs of cytoskeletal proteins not previously documented in sea urchins. To illustrate the varying degree of sequence diversity that exists within cytoskeletal gene families, we conducted an analysis of genes encoding actins, specific actin-binding proteins, myosins, tubulins, kinesins, dyneins, specific microtubule-associated proteins, and intermediate filaments. We conducted ontological analysis of select genes to better understand the relatedness of urchin cytoskeletal genes to those of other deuterostomes. We analyzed developmental expression (EST) data to confirm the existence of select gene models and to understand their differential expression during various stages of early development.
???displayArticle.pubmedLink??? 17027957
???displayArticle.pmcLink??? PMC2590651
???displayArticle.link??? Dev Biol
???displayArticle.grants??? [+]
GM30401 NIGMS NIH HHS , R37 GM030401 NIGMS NIH HHS , R01 GM030401 NIGMS NIH HHS , GM58231 NIGMS NIH HHS , R01 GM058231 NIGMS NIH HHS
Genes referenced: LOC100887844 LOC115919910 LOC590297
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