ECB-ART-39700
Dev Dyn
2006 Apr 01;2354:1042-52. doi: 10.1002/dvdy.20695.
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Calcium-responsive contractility during fertilization in sea urchin eggs.
Stack C
,
Lucero AJ
,
Shuster CB
.
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Fertilization triggers a reorganization of oocyte cytoskeleton, and in sea urchins, there is a dramatic increase in cortical F-actin. However, the role that myosin II plays during fertilization remains largely unexplored. Myosin II is localized to the cortical cytoskeleton both before and after fertilization and to examine myosin II contractility in living cells, Lytechinus pictus eggs were observed by time-lapse microscopy. Upon sperm binding, a cell surface deflection traversed the egg that was followed by and dependent on the calcium wave. The calcium-dependence of surface contractility could be reproduced in unfertilized eggs, where mobilization of intracellular calcium in unfertilized eggs under compression resulted in a marked contractile response. Lastly, inhibition of myosin II delayed absorption of the fertilization cone, suggesting that myosin II not only responds to the same signals that activate eggs but also participates in the remodeling of the cortical actomyosin cytoskeleton during the first zygotic cell cycle.
???displayArticle.pubmedLink??? 16470603
???displayArticle.pmcLink??? PMC2566787
???displayArticle.link??? Dev Dyn
???displayArticle.grants??? [+]
GM 61222 NIGMS NIH HHS , U54 CA132383 NCI NIH HHS , S06 GM008136 NIGMS NIH HHS , S06 GM 08136 NIGMS NIH HHS , S06 GM008136-310052 NIGMS NIH HHS , S06 GM008136-320052 NIGMS NIH HHS , R25 GM061222-06 NIGMS NIH HHS , R25 GM061222 NIGMS NIH HHS , U56 CA096286 NCI NIH HHS
Genes referenced: cep152 LOC100887844 LOC115919910 LOC590297
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