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Mol Biol Cell
2005 Oct 01;1610:4485-94. doi: 10.1091/mbc.e05-04-0305.
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Induction of cytokinesis is independent of precisely regulated microtubule dynamics.
Strickland LI
,
Donnelly EJ
,
Burgess DR
.
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Astral microtubules (MTs) emanating from the mitotic apparatus (MA) during anaphase are required for stimulation of cytokinesis in eggs. We have used green fluorescent protein-labeled EB1 to observe MT dynamics during mitosis and cytokinesis in normal sea urchin eggs. Analysis of astral MT growth rates during anaphase shows that MTs contact the polar cortex earlier than the equatorial cortex after anaphase onset but that a normal cleavage furrow is not induced until contact with MTs has been achieved throughout the cortex. To assess the role of MT dynamics in initiation of cytokinesis, we used a collection of small molecule drugs to affect dynamics. Hexylene glycol resulted in rapid astral elongation due to decreased MT catastrophe and precocious furrowing. Taxol suppressed MT dynamics but did not inhibit furrow induction when the MA was manipulated toward the cortex. Urethane resulted in short, highly dynamic astral MTs with increased catastrophe that also stimulated furrowing upon being brought into proximity to the cortex. Our findings indicate that astral MT contact with the cortex is necessary for furrow initiation but that the dynamic state of astral MTs does not affect their competency to stimulate furrowing.
Akhmanova,
Microtubule plus-end-tracking proteins: mechanisms and functions.
2005, Pubmed
Akhmanova,
Microtubule plus-end-tracking proteins: mechanisms and functions.
2005,
Pubmed
Asnes,
Cell cleavage. Ultrastructural evidence against equatorial stimulation by aster microtubules.
1979,
Pubmed
,
Echinobase
Balasubramanian,
Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells.
2004,
Pubmed
Burgess,
Site selection for the cleavage furrow at cytokinesis.
2005,
Pubmed
Canman,
Determining the position of the cell division plane.
2003,
Pubmed
Carvalho,
Surfing on microtubule ends.
2003,
Pubmed
Checchi,
Microtubule-interacting drugs for cancer treatment.
2003,
Pubmed
Conrad,
Effect of hexylene glycol-altered microtubule distributions on cytokinesis and polar lobe formation in fertilized eggs of Ilyanassa obsoleta.
1994,
Pubmed
D'Avino,
Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy.
2005,
Pubmed
De Brabander,
Microtubule dynamics during the cell cycle: the effects of taxol and nocodazole on the microtubule system of Pt K2 cells at different stages of the mitotic cycle.
1986,
Pubmed
Dechant,
Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation.
2003,
Pubmed
Galjart,
A plus-end raft to control microtubule dynamics and function.
2003,
Pubmed
Glotzer,
The molecular requirements for cytokinesis.
2005,
Pubmed
Gundersen,
Microtubule capture: IQGAP and CLIP-170 expand the repertoire.
2002,
Pubmed
Gundersen,
Cortical control of microtubule stability and polarization.
2004,
Pubmed
Hamaguchi,
Displacement of cleavage plane in the sea urchin egg by locally applied taxol.
1998,
Pubmed
,
Echinobase
Lee,
Positioning of the mitotic spindle by a cortical-microtubule capture mechanism.
2000,
Pubmed
Mallavarapu,
A switch in microtubule dynamics at the onset of anaphase B in the mitotic spindle of Schizosaccharomyces pombe.
1999,
Pubmed
Mimori-Kiyosue,
The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules.
2000,
Pubmed
Morrison,
EB 1 immunofluorescence reveals an increase in growing astral microtubule length and number during anaphase in NRK-52E cells.
2001,
Pubmed
Oka,
Change in the heterogeneous distribution of tubulin isotypes in mitotic microtubules of the sea urchin egg by treatment with microtubule depolymerizing or stabilizing drugs.
1991,
Pubmed
,
Echinobase
Piehl,
Organization and dynamics of growing microtubule plus ends during early mitosis.
2003,
Pubmed
Piehl,
Centrosome maturation: measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1.
2004,
Pubmed
RAPPAPORT,
DURATION OF STIMULUS AND LATENT PERIODS PRECEDING FURROW FORMATION IN SAND DOLLAR EGGS.
1965,
Pubmed
,
Echinobase
Rappaport,
Aster-equatorial surface relations and furrow establishment.
1969,
Pubmed
Rappaport,
Division of constricted and urethane-treated sand dollar eggs: a test of the polar stimulation hypothesis.
1984,
Pubmed
,
Echinobase
Rusan,
Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport.
2002,
Pubmed
Salmon,
Taxol stabilization of mitotic spindle microtubules: analysis using calcium-induced depolymerization.
1984,
Pubmed
,
Echinobase
Shuster,
Parameters that specify the timing of cytokinesis.
1999,
Pubmed
,
Echinobase
Shuster,
Transitions regulating the timing of cytokinesis in embryonic cells.
2002,
Pubmed
,
Echinobase
Snyder,
Analysis of spindle microtubule organization in untreated and taxol-treated PtK1 cells.
1993,
Pubmed
Strickland,
Light microscopy of echinoderm embryos.
2004,
Pubmed
,
Echinobase
Tirnauer,
EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability.
2000,
Pubmed
Tirnauer,
EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules.
2002,
Pubmed
Tirnauer,
Microtubule plus-end dynamics in Xenopus egg extract spindles.
2004,
Pubmed
Vaughan,
Surfing, regulating and capturing: are all microtubule-tip-tracking proteins created equal?
2004,
Pubmed
White,
On the mechanisms of cytokinesis in animal cells.
1983,
Pubmed
Yvon,
Taxol suppresses dynamics of individual microtubules in living human tumor cells.
1999,
Pubmed
Zhai,
Kinetochore microtubule dynamics and the metaphase-anaphase transition.
1995,
Pubmed
