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Dev Genes Evol
2005 Aug 01;2158:410-22. doi: 10.1007/s00427-005-0489-6.
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Structure, expression, and transcriptional regulation of the Strongylocentrotus franciscanus spec gene family encoding intracellular calcium-binding proteins.
Villinski JT
,
Kiyama T
,
Dayal S
,
Zhang N
,
Liang S
,
Klein WH
.
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The mechanisms by which gene expression patterns emerge during evolution are poorly understood. The sea urchin spec genes offer a useful means to investigate evolutionary mechanisms. Genes of the spec family from Strongylocentrotus purpuratus and Lytechinus pictus have identical patterns of aboral ectoderm-specific expression but exhibit species-specific differences in copy number, genomic structure, temporal expression, and cis-regulatory architecture. Here, we identify spec genes from a phylogenetic intermediate, Strongylocentrotus franciscanus, to gain insight into the evolution of the spec gene family and its transcriptional regulation. We identified two spec genes in the S. franciscanus genome, sfspec1a and sfspec1b, that were orthologous to spec1 from S. purpuratus. sfspec1b transcripts began to accumulate at the blastula stage and became progressively more abundant; this was reminiscent of spec expression in L. pictus but different from that in S. purpuratus. As expected, sfspec1b expression was restricted to aboral ectoderm cells. The six-exon structure of the sfspec1b genomic locus was identical to that of the S. purpuratus spec genes and was bounded by two repeat-spacer-repeat (RSR) repetitive sequence elements, which are conserved features of S. purpuratus spec genes and function as transcriptional enhancers. The enhancer activity of the sfspec1b RSRs was comparable to that of their S. purpuratus counterparts, although the placement and orientation of crucial cis-regulatory elements within the RSRs differed. We discovered a spec gene in S. franciscanus that was only distantly related to other spec genes but was highly conserved in S. purpuratus. Unexpectedly, this gene was expressed exclusively in endoderm lineages. Our results show that the evolution of spec cis-regulatory elements is highly dynamic and that substantial alterations can occur when maintaining or grossly modifying gene expression patterns.
Akasaka,
Introduction of DNA into sea urchin eggs by particle gun.
1995, Pubmed,
Echinobase
Akasaka,
Introduction of DNA into sea urchin eggs by particle gun.
1995,
Pubmed
,
Echinobase
Angerer,
Sea urchin goosecoid function links fate specification along the animal-vegetal and oral-aboral embryonic axes.
2001,
Pubmed
,
Echinobase
Brandhorst,
Molecular patterning along the sea urchin animal-vegetal axis.
2002,
Pubmed
,
Echinobase
Britten,
Gene regulation for higher cells: a theory.
1969,
Pubmed
Carpenter,
Novel proteins belonging to the troponin C superfamily are encoded by a set of mRNAs in sea urchin embryos.
1984,
Pubmed
,
Echinobase
Dayal,
Creation of cis-regulatory elements during sea urchin evolution by co-option and optimization of a repetitive sequence adjacent to the spec2a gene.
2004,
Pubmed
,
Echinobase
Fang,
Evolution of actin gene families of sea urchins.
1994,
Pubmed
,
Echinobase
Gan,
An orthodenticle-related protein from Strongylocentrotus purpuratus.
1995,
Pubmed
,
Echinobase
Gan,
Regulatory elements from the related spec genes of Strongylocentrotus purpuratus yield different spatial patterns with a lacZ reporter gene.
1990,
Pubmed
,
Echinobase
Gan,
Repetitive DNA sequences linked to the sea urchin spec genes contain transcriptional enhancer-like elements.
1990,
Pubmed
,
Echinobase
Gan,
A positive cis-regulatory element with a bicoid target site lies within the sea urchin Spec2a enhancer.
1993,
Pubmed
,
Echinobase
Hardin,
Structure of the Spec1 gene encoding a major calcium-binding protein in the embryonic ectoderm of the sea urchin, Strongylocentrotus purpuratus.
1985,
Pubmed
,
Echinobase
Hardin,
Spec2 genes of Strongylocentrotus purpuratus. Structure and differential expression in embryonic aboral ectoderm cells.
1988,
Pubmed
,
Echinobase
Howard,
cis-Regulatory control circuits in development.
2004,
Pubmed
,
Echinobase
Kissinger,
Evolutionary changes in sites and timing of actin gene expression in embryos of the direct- and indirect-developing sea urchins, Heliocidaris erythrogramma and H. tuberculata.
1998,
Pubmed
,
Echinobase
Kiyama,
Strongylocentrotus purpuratus transcription factor GATA-E binds to and represses transcription at an Otx-Goosecoid cis-regulatory element within the aboral ectoderm-specific spec2a enhancer.
2005,
Pubmed
,
Echinobase
Kumar,
MEGA2: molecular evolutionary genetics analysis software.
2001,
Pubmed
Lee,
Molecular phylogenies and divergence times of sea urchin species of Strongylocentrotidae, Echinoida.
2003,
Pubmed
,
Echinobase
Li,
Ectoderm gene activation in sea urchin embryos mediated by the CCAAT-binding factor.
2002,
Pubmed
,
Echinobase
Li,
Two Otx proteins generated from multiple transcripts of a single gene in Strongylocentrotus purpuratus.
1997,
Pubmed
,
Echinobase
Littlewood,
A combined morphological and molecular phylogeny for sea urchins (Echinoidea: Echinodermata).
1995,
Pubmed
,
Echinobase
Mao,
Multiple Otx binding sites required for expression of the Strongylocentrotus purpuratus Spec2a gene.
1994,
Pubmed
,
Echinobase
Mao,
Altering cell fates in sea urchin embryos by overexpressing SpOtx, an orthodenticle-related protein.
1996,
Pubmed
,
Echinobase
Ransick,
New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization.
2002,
Pubmed
,
Echinobase
Shoguchi,
In situ screening for genes expressed preferentially in secondary mesenchyme cells of sea urchin embryos.
2002,
Pubmed
,
Echinobase
Tomlinson,
Temporal and spatial transcriptional regulation of the aboral ectoderm-specific Spec genes during sea urchin embryogenesis.
1990,
Pubmed
,
Echinobase
Xiang,
Structure and promoter activity of the LpS1 genes of Lytechinus pictus. Duplicated exons account for LpS1 proteins with eight calcium binding domains.
1991,
Pubmed
,
Echinobase
Xiang,
Tandem duplication and divergence of a sea urchin protein belonging to the troponin C superfamily.
1988,
Pubmed
,
Echinobase
Yuh,
Correct Expression of spec2a in the sea urchin embryo requires both Otx and other cis-regulatory elements.
2001,
Pubmed
,
Echinobase
