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RNA 2005 May 01;115:646-56. doi: 10.1261/rna.7198205.
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Seawi--a sea urchin piwi/argonaute family member is a component of MT-RNP complexes.

Rodriguez AJ , Seipel SA , Hamill DR , Romancino DP , DI Carlo M , Suprenant KA , Bonder EM .

The piwi/argonaute family of proteins is involved in key developmental processes such as stem cell maintenance and axis specification through molecular mechanisms that may involve RNA silencing. Here we report on the cloning and characterization of the sea urchin piwi/argonaute family member seawi. Seawi is a major component of microtubule-ribonucleoprotein (MT-RNP) complexes isolated from two different species of sea urchin, Strongylocentrotus purpuratus and Paracentrotus lividus. Seawi co-isolates with purified ribosomes, cosediments with 80S ribosomes in sucrose density gradients, and binds microtubules. Seawi possesses the RNA binding motif common to piwi family members and binds P. lividus bep4 mRNA, a transcript that co-isolates with MT-RNP complexes and whose translation product has been shown to play a role in patterning the animal-vegetal axis. Indirect immunofluorescence studies localized seawi to the cortex of unfertilized eggs within granule-like particles, the mitotic spindle during cell division, and the small micromeres where its levels were enriched during the early cleavage stage. Lastly, we discuss how seawi, as a piwi/argonaute family member, may play a fundamentally important role in sea urchin animal-vegetal axis formation and stem cell maintenance.

PubMed ID: 15840816
PMC ID: PMC1370751
Article link: RNA

Genes referenced: LOC100887844 LOC115919910 piwil3
Antibodies: piwil3 Ab1 piwil3 Ab2

References [+] :
Angerer, Animal-vegetal axis patterning mechanisms in the early sea urchin embryo. 2000, Pubmed, Echinobase