Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Angiogenic factors in human proliferative sickle cell retinopathy.
Cao J
,
Mathews MK
,
McLeod DS
,
Merges C
,
Hjelmeland LM
,
Lutty GA
.
???displayArticle.abstract???
BACKGROUND/AIMS: Preretinal neovascular formations called sea fans develop at the border of non-perfused peripheral retina in sickle cell retinopathy. Angiogenic factors which could contribute to their development, however, have not been examined previously. The objective of this study was to determine immunohistochemically if vascular endothelial growth factor (VEGF) or basic fibroblast growth factor (bFGF) were associated with sea fan formations.
METHODS: Immunohistochemistry on cryosections was used to localise bFGF, VEGF, heparan sulphate proteoglycan, human serum albumin, collagens IV and II, and von Willebrand factor in tissue from five sickle cell and one control subject.
RESULTS: The greatest immunoreactivity for VEGF and bFGF was in the feeder and preretinal vessels of sea fans (p<0.01). The most prominent reaction product was localised to vascular endothelial cells. In retinal vessels, VEGF and bFGF immunoreactivities were greater in sickle cell subjects (both proliferative and non-proliferative) than in the control subject (p<0.01 and p<0.02 respectively). In the sickle cell retina, no angiogenic factor immunoreactivity was detected in non-perfused periphery and there was no significant difference in bFGF or VEGF immunoreactivity between perfused retina and the border of perfused and non-perfused areas.
CONCLUSION: Our results demonstrate for the first time that VEGF and bFGF are associated with sea fan formations in sickle cell retinopathy. Both factors may function in an autocrine manner because immunoreactivity for these factors was greater within the neovascularisation than in adjacent retina.
Aiello,
Hypoxic regulation of vascular endothelial growth factor in retinal cells.
1995, Pubmed
Aiello,
Hypoxic regulation of vascular endothelial growth factor in retinal cells.
1995,
Pubmed
Amin,
Vascular endothelial growth factor is present in glial cells of the retina and optic nerve of human subjects with nonproliferative diabetic retinopathy.
1997,
Pubmed
Bost,
Coexpression of FGF-5 and bFGF by the retinal pigment epithelium in vitro.
1992,
Pubmed
Boulton,
VEGF localisation in diabetic retinopathy.
1998,
Pubmed
Chen,
Localisation of vascular endothelial growth factor and its receptors to cells of vascular and avascular epiretinal membranes.
1997,
Pubmed
Dvorak,
Distribution of vascular permeability factor (vascular endothelial growth factor) in tumors: concentration in tumor blood vessels.
1991,
Pubmed
Fukushima,
Intrachoroidal microvascular abnormality: a previously unrecognized form of choroidal neovascularization.
1997,
Pubmed
Gaudry,
Intracellular pool of vascular endothelial growth factor in human neutrophils.
1997,
Pubmed
Hanneken,
Altered distribution of basic fibroblast growth factor in diabetic retinopathy.
1991,
Pubmed
Healy,
Density-dependent accumulation of basic fibroblast growth factor in the subendothelial matrix.
1992,
Pubmed
Ljubimov,
Basement membrane abnormalities in human eyes with diabetic retinopathy.
1996,
Pubmed
Lutty,
Localization of vascular endothelial growth factor in human retina and choroid.
1996,
Pubmed
Lutty,
Heterogeneity in localization of isoforms of TGF-beta in human retina, vitreous, and choroid.
1993,
Pubmed
Lutty,
Immunohistochemical insights into sickle cell retinopathy.
1994,
Pubmed
Mathews,
Vascular endothelial growth factor and vascular permeability changes in human diabetic retinopathy.
1997,
Pubmed
McLeod,
Dual-perspective analysis of vascular formations in sickle cell retinopathy.
1993,
Pubmed
McLeod,
Enhanced expression of intracellular adhesion molecule-1 and P-selectin in the diabetic human retina and choroid.
1995,
Pubmed
Nauck,
Induction of vascular endothelial growth factor by platelet-activating factor and platelet-derived growth factor is downregulated by corticosteroids.
1997,
Pubmed
Page,
Antigenic heterogeneity of vascular endothelium.
1992,
Pubmed
Pe'er,
Hypoxia-induced expression of vascular endothelial growth factor by retinal cells is a common factor in neovascularizing ocular diseases.
1995,
Pubmed
Shima,
Hypoxic induction of vascular endothelial growth factor (VEGF) in human epithelial cells is mediated by increases in mRNA stability.
1995,
Pubmed
Shima,
Hypoxic induction of endothelial cell growth factors in retinal cells: identification and characterization of vascular endothelial growth factor (VEGF) as the mitogen.
1995,
Pubmed
Shima,
Cloning and mRNA expression of vascular endothelial growth factor in ischemic retinas of Macaca fascicularis.
1996,
Pubmed
Shweiki,
Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis.
1992,
Pubmed
Simorre-Pinatel,
Vasculotropin-VEGF stimulates retinal capillary endothelial cells through an autocrine pathway.
1994,
Pubmed
Stavri,
Hypoxia and platelet-derived growth factor-BB synergistically upregulate the expression of vascular endothelial growth factor in vascular smooth muscle cells.
1995,
Pubmed
Taichman,
Human neutrophils secrete vascular endothelial growth factor.
1997,
Pubmed
Talbot,
Sickle cell retinopathy in Jamaican children: further observations from a cohort study.
1988,
Pubmed
Vinores,
Localization of blood-retinal barrier breakdown in human pathologic specimens by immunohistochemical staining for albumin.
1990,
Pubmed
