Carroll DJ , Ramarao CS , Mehlmann LM , Roche S , Terasaki M , Jaffe LA .

	Figure 2. Delay of Ca2+ release during fertilization of starfish eggs injected with PLCγSH2(N+C) protein. Eggs were co-injected with 10 μM Ca-green dextran; photomultiplier current indicating Ca-green fluorescence is shown as a function of time. Each fluorescence trace was normalized to the fluorescence of the unfertilized egg. Arrows indicate the time of sperm addition (1:5,000 dilution of the suspension from the testis). (A) An egg that was injected with 1,100 μg/ml of the GST control protein. The asterisk indicates the action potential. (B and C) Eggs that were injected with 105 (B) or 900 (C) μg/ml of the PLCγSH2 (N+C) protein. In C, the lower line shows the continuation of the same record. (D) An egg that was injected with 1,000 μg/ml of the SHP2-SH2(N+C) control protein.
	Figure 3. Ca2+ imaging during fertilization of starfish eggs injected with PLCγSH2(N+C) or GST proteins. The control egg on the left was injected with 190 μg/ml GST and 10 μM Ca-green dextran. The egg on the right was injected with 130 μg/ml PLCγSH2(N+C) and 10 μM Ca-green dextran. Fluorescence intensity (0–255) is displayed using the 32-color look-up table of NIH Image (increasing values correspond to purple, blue, green, yellow, orange, red). Images are shown at 3-s intervals; the sequence is shown reading down each column, starting at the upper left. Sperm (1:5,000 dilution of the suspension from the testis) were added to the recording chamber 14 s before the first image. In the second image in the first column, an action potential occurred in both eggs (increased fluorescence at the egg surface). In the third image in the first column, the Ca2+ wave is starting in the control egg. In the first image in the fourth column, a local Ca2+ rise occurs in the PLCγSH2(N+C)-injected egg but does not propagate across the egg. In the last image in the fourth column, another Ca2+ rise occurs in the PLCγSH2(N+C)-injected egg, and a Ca2+ wave propagates across the egg in the fifth column. The difference in fluorescence intensity between the egg cortex and interior is due at least in part to greater absorbance of light in the center of the egg where the specimen is thicker; it is also possible that there is a real difference in Ca2+ activity between the cortex and interior. A Quicktime movie sequence from this experiment is available at: http://www.uchc.edu/∼terasaki/data/sh2.html. Bar, 200 μm.
	Figure 4. Inhibition of Ca2+ release during fertilization of starfish eggs injected with PLCγSH2(N+C) protein and exposed to a low concentration of sperm (1:50,000 dilution of the suspension from the testis). Photomultiplier current as a function of time. (A) An egg that was injected with 1,000 μg/ml of the SHP2-SH2(N+C) control protein. (B) An egg that was injected with 1,000 μg/ml of the PLCγSH2(N+C) protein. Arrows indicate the time of sperm addition. The lower lines show the continuation of the same record. (C and D) Images of eggs injected with 1,000 μg/ml SHP2-SH2(N+C) and 1,000 μg/ml PLCγ-SH2(N+C) and inseminated with a 1:50,000 sperm dilution; images were taken at the time of first cleavage in uninjected eggs, using the scanning transmission mode of the BioRad confocal microscope. (C) The SHP2-SH2(N+C)-injected egg showed a Ca-green record like that in A, elevated a normal fertilization envelope, and cleaved normally. Photographed at 3.0 h after insemination. (D) The PLCγSH2(N+C)-injected egg showed a Ca-green record like that in B and did not elevate a fertilization envelope or cleave; however, the presence of two pronuclei in the cytoplasm indicated that it was fertilized. Photographed at 2.6 h after insemination. These pronuclei were similar in appearance to those described by Sluder et al. (1989) for normal starfish fertilization, although the time course of their development was not compared with that in control eggs. Asterisks indicate pronuclei; “oil” indicates the oil drop introduced during microinjection. Bar, 100 μm.
	Figure 5. Normal Ca2+ release during fertilization of starfish eggs injected with a point-mutated PLCγ SH2(N+C) protein. Photomultiplier current as a function of time. Arrows indicate the time of sperm addition (1:5,000 dilution of the suspension from the testis). (A) An egg that was injected with 260 μg/ml of the PLCγ SH2(N+C)-mut protein. (B) An egg that was injected with 220 μg/ml of the PLCγ SH2(N+C)-wt protein.
	Figure 6. Normal Ca2+ release in starfish eggs injected with the PLCγSH2(N+C) protein and treated with IP3 or serotonin. (A and B) Photomultiplier records from oocytes that were injected with Ca-green dextran and for B, 1,000 μg/ml PLCγSH2(N+C), and then matured and injected with 1% of the cell volume of 1 μM IP3 (arrows). (C and D) Prophase-stage oocytes were injected with serotonin 2c receptor RNA (30 pg/oocyte). 24–27 h later the oocytes were injected with 10 μM Ca-green dextran and for D, 1,000 μg/ml PLCγSH2(N+C), and then treated with 1-methyladenine to cause maturation to the first meiotic metaphase stage. Serotonin (1 μM) was applied (arrows), while recording Ca-green fluorescence. Photomultiplier current indicating Ca-green fluorescence is shown as a function of time.

Abassi, Tyrosine phosphorylation of the egg receptor for sperm at fertilization. 1994, Pubmed, Echinobase

Abassi, Tyrosine phosphorylation of the egg receptor for sperm at fertilization. 1994, Pubmed , Echinobase
Adachi, Mammalian SH2-containing protein tyrosine phosphatases. 1996, Pubmed
Anderson, Binding of SH2 domains of phospholipase C gamma 1, GAP, and Src to activated growth factor receptors. 1990, Pubmed
Banno, Thrombin-mediated phosphoinositide hydrolysis in Chinese hamster ovary cells overexpressing phospholipase C-delta 1. 1994, Pubmed
Baxter, 5-HT2 receptor subtypes: a family re-united? 1995, Pubmed
Bezprozvanny, Activation of the calcium release channel (ryanodine receptor) by heparin and other polyanions is calcium dependent. 1993, Pubmed
Chiba, Development of calcium release mechanisms during starfish oocyte maturation. 1990, Pubmed , Echinobase
Ciapa, Two phases of inositol polyphosphate and diacylglycerol production at fertilisation. 1986, Pubmed , Echinobase
Ciapa, A rapid change in phosphorylation on tyrosine accompanies fertilization of sea urchin eggs. 1991, Pubmed , Echinobase
Claesson-Welsh, Platelet-derived growth factor receptor signals. 1994, Pubmed
Crossley, Guanosine 5'-thiotriphosphate may stimulate phosphoinositide messenger production in sea urchin eggs by a different route than the fertilizing sperm. 1991, Pubmed , Echinobase
Dale, Injection of a soluble sperm fraction into sea-urchin eggs triggers the cortical reaction. 1985, Pubmed , Echinobase
Dupont, Phospholipase C in mouse oocytes: characterization of beta and gamma isoforms and their possible involvement in sperm-induced Ca2+ spiking. 1996, Pubmed
Feng, SH2-containing phosphotyrosine phosphatase as a target of protein-tyrosine kinases. 1993, Pubmed
Galione, Redundant mechanisms of calcium-induced calcium release underlying calcium waves during fertilization of sea urchin eggs. 1993, Pubmed , Echinobase
Gish, Biochemical analysis of SH2 domain-mediated protein interactions. 1995, Pubmed
Hagiwara, Electrical properties of egg cell membranes. 1979, Pubmed
Hamaguchi, Distribution of fluorescently labeled tubulin injected into sand dollar eggs from fertilization through cleavage. 1985, Pubmed , Echinobase
Hernández-Sotomayor, Non-catalytic activation of phospholipase C-gamma 1 in vitro by epidermal growth factor receptor. 1993, Pubmed
Huang, An SH3 domain is required for the mitogenic activity of microinjected phospholipase C-gamma 1. 1995, Pubmed
Itoh, Beta-tubulin binds Src homology 2 domains through a region different from the tyrosine-phosphorylated protein-recognizing site. 1996, Pubmed
Iwashima, Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. 1994, Pubmed
Jaffe, Fast block to polyspermy in sea urchin eggs is electrically mediated. 1976, Pubmed , Echinobase
Jaffe, First messengers at fertilization. 1990, Pubmed , Echinobase
Julius, Molecular characterization of a functional cDNA encoding the serotonin 1c receptor. 1988, Pubmed
Kiehart, Microinjection of echinoderm eggs: apparatus and procedures. 1982, Pubmed , Echinobase
Kim, PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254. 1991, Pubmed
Kinsey, Differential phosphorylation of a 57-KDa protein tyrosine kinase during egg activation. 1995, Pubmed , Echinobase
Kinsey, Biphasic activation of Fyn kinase upon fertilization of the sea urchin egg. 1996, Pubmed , Echinobase
Kishimoto, Microinjection and cytoplasmic transfer in starfish oocytes. 1986, Pubmed , Echinobase
Kline, A calcium-activated sodium conductance contributes to the fertilization potential in the egg of the nemertean worm Cerebratulus lacteus. 1986, Pubmed
Kline, Calcium-dependent events at fertilization of the frog egg: injection of a calcium buffer blocks ion channel opening, exocytosis, and formation of pronuclei. 1988, Pubmed
Kline, Fertilization events induced by neurotransmitters after injection of mRNA in Xenopus eggs. 1988, Pubmed
Koch, SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins. 1991, Pubmed
Larose, Construction of an SH2 domain-binding site with mixed specificity. 1995, Pubmed
Lawrence, Sperm-egg fusion is the prelude to the initial Ca2+ increase at fertilization in the mouse. 1997, Pubmed
Lee, Calcium mobilization by dual receptors during fertilization of sea urchin eggs. 1993, Pubmed , Echinobase
Longo, Stages leading to and following fusion of sperm and egg plasma membranes. 1994, Pubmed , Echinobase
Mabuchi, Alpha-actinin from sea urchin eggs: biochemical properties, interaction with actin, and distribution in the cell during fertilization and cleavage. 1985, Pubmed , Echinobase
Mayer, Point mutations in the abl SH2 domain coordinately impair phosphotyrosine binding in vitro and transforming activity in vivo. 1992, Pubmed
McCulloh, Fusion of membranes during fertilization. Increases of the sea urchin egg's membrane capacitance and membrane conductance at the site of contact with the sperm. 1992, Pubmed , Echinobase
McDougall, Thimerosal reveals calcium-induced calcium release in unfertilised sea urchin eggs. 1993, Pubmed , Echinobase
Miyazaki, Fast polyspermy block and activation potential. Correlated changes during oocyte maturation of a starfish. 1979, Pubmed , Echinobase
Miyazaki, Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs. 1992, Pubmed
Miyazaki, Inositol 1,4,5-trisphosphate-induced calcium release and guanine nucleotide-binding protein-mediated periodic calcium rises in golden hamster eggs. 1988, Pubmed
Miyazaki, Action potential and non-linear current-voltage relation in starfish oocytes. 1975, Pubmed , Echinobase
Mohri, Effect on sperm-induced activation current and increase of cytosolic Ca2+ by agents that modify the mobilization of [Ca2+]i. I. Heparin and pentosan polysulfate. 1995, Pubmed , Echinobase
Moore, Effects of protein tyrosine kinase inhibitors on egg activation and fertilization-dependent protein tyrosine kinase activity. 1995, Pubmed , Echinobase
Moore, Roles of heterotrimeric and monomeric G proteins in sperm-induced activation of mouse eggs. 1994, Pubmed
Moore, Identification of an abl-related protein tyrosine kinase in the cortex of the sea urchin egg: possible role at fertilization. 1994, Pubmed , Echinobase
Nuccitelli, The sperm-induced Ca2+ wave following fertilization of the Xenopus egg requires the production of Ins(1, 4, 5)P3. 1993, Pubmed
Park, Cloning, sequencing, expression, and Gq-independent activation of phospholipase C-beta 2. 1992, Pubmed
Parrington, Calcium oscillations in mammalian eggs triggered by a soluble sperm protein. 1996, Pubmed
Pawson, Protein modules and signalling networks. 1995, Pubmed
Rhee, Regulation of inositol phospholipid-specific phospholipase C isozymes. 1992, Pubmed
Roche, Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent pathways. 1996, Pubmed
Rotin, SH2 domains prevent tyrosine dephosphorylation of the EGF receptor: identification of Tyr992 as the high-affinity binding site for SH2 domains of phospholipase C gamma. 1992, Pubmed
Sette, Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa. 1997, Pubmed
Shen, Sources of calcium in sea urchin eggs during the fertilization response. 1993, Pubmed , Echinobase
Shilling, Evidence for both tyrosine kinase and G-protein-coupled pathways leading to starfish egg activation. 1994, Pubmed , Echinobase
Shilling, Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor. 1990, Pubmed , Echinobase
Sillman, Association of p72syk with the src homology-2 (SH2) domains of PLC gamma 1 in B lymphocytes. 1995, Pubmed
Sluder, Centrosome inheritance in starfish zygotes: selective loss of the maternal centrosome after fertilization. 1989, Pubmed , Echinobase
Stricker, Intracellular injections of a soluble sperm factor trigger calcium oscillations and meiotic maturation in unfertilized oocytes of a marine worm. 1997, Pubmed
Stricker, Calcium dynamics during starfish oocyte maturation and fertilization. 1994, Pubmed , Echinobase
Wahl, Growth factor stimulation of phospholipase C-gamma 1 activity. Comparative properties of control and activated enzymes. 1992, Pubmed
Williams, Role of G proteins in mouse egg activation: stimulatory effects of acetylcholine on the ZP2 to ZP2f conversion and pronuclear formation in eggs expressing a functional m1 muscarinic receptor. 1992, Pubmed
Yim, Highly polarized EGF receptor tyrosine kinase activity initiates egg activation in Xenopus. 1994, Pubmed