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J Cell Biol
1996 Feb 01;1323:371-80. doi: 10.1083/jcb.132.3.371.
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Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex.
Wedaman KP
,
Meyer DW
,
Rashid DJ
,
Cole DG
,
Scholey JM
.
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The heterotrimeric kinesin-II holoenzyme purified from sea urchin (Strongylocentrotus purpuratus) eggs is assembled from two heterodimerized kinesin-related motor subunits of known sequence, together with a third, previously uncharacterized 115-kD subunit, SpKAP115. Using monospecific anti-SpKAP115 antibodies we have accomplished the molecular cloning and sequencing of the SpKAP115 subunit. The deduced sequence predicts a globular 95-kD non-motor "accessory" polypeptide rich in alpha-helical segments that are generally not predicted to form coiled coils. Electron microscopy of individual rotary shadowed kinesin-II holoenzymes also suggests that SpKAP115 is globular, with a somewhat asymmetric morphology. Moreover, the SpKAP115 subunit lies at one end of the 51-nm-long kinesin-II complex, being separated from the two presumptive motor domains by a approximately 26-nm-long rod, in a manner similar to the light chains (KLCs) of kinesin itself. This indicates that SpKAP115 and the KLCs may have analogous functions, yet SpKAP115 does not display significant sequence similarity with the KLCs. The results show that kinesin and kinesin-II are assembled from highly divergent accessory polypeptides together with kinesin related motor subunits (KRPs) containing conserved motor domains linked to divergent tails. Despite the lack of sequence conservation outside the motor domains, there is striking conservation of the ultrastructure of the kinesin and kinesin-II holoenzymes.
Aizawa,
Kinesin family in murine central nervous system.
1992, Pubmed
Aizawa,
Kinesin family in murine central nervous system.
1992,
Pubmed
Biou,
Secondary structure prediction: combination of three different methods.
1988,
Pubmed
Bloom,
Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
1988,
Pubmed
Bloom,
Motor proteins. 1: kinesins.
1994,
Pubmed
Brady,
A novel brain ATPase with properties expected for the fast axonal transport motor.
1985,
Pubmed
Buster,
Purification and assay of kinesin from sea urchin eggs and early embryos.
1991,
Pubmed
,
Echinobase
Cohn,
Quantitative analysis of sea urchin egg kinesin-driven microtubule motility.
1989,
Pubmed
,
Echinobase
Cole,
Structural variations among the kinesins.
1995,
Pubmed
Cole,
Novel heterotrimeric kinesin-related protein purified from sea urchin eggs.
1993,
Pubmed
,
Echinobase
Cole,
A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos.
1994,
Pubmed
Cole,
Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
1992,
Pubmed
,
Echinobase
Cole,
Purification of kinesin-related protein complexes from eggs and embryos.
1995,
Pubmed
,
Echinobase
Craig,
Light-chain phosphorylation controls the conformation of vertebrate non-muscle and smooth muscle myosin molecules.
1983,
Pubmed
Cyr,
Molecular genetics of kinesin light chains: generation of isoforms by alternative splicing.
1991,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Gauger,
The Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain.
1993,
Pubmed
Goldstein,
With apologies to scheherazade: tails of 1001 kinesin motors.
1993,
Pubmed
Hackney,
Kinesin undergoes a 9 S to 6 S conformational transition.
1992,
Pubmed
Hackney,
Characterization of alpha 2 beta 2 and alpha 2 forms of kinesin.
1991,
Pubmed
,
Echinobase
Henson,
Immunolocalization of the heterotrimeric kinesin-related protein KRP(85/95) in the mitotic apparatus of sea urchin embryos.
1995,
Pubmed
,
Echinobase
Hirokawa,
Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
1989,
Pubmed
Hisanaga,
The molecular structure of adrenal medulla kinesin.
1989,
Pubmed
Johnson,
Light chains of sea urchin kinesin identified by immunoadsorption.
1990,
Pubmed
,
Echinobase
Kondo,
KIF3A is a new microtubule-based anterograde motor in the nerve axon.
1994,
Pubmed
Kuznetsov,
The quaternary structure of bovine brain kinesin.
1988,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed
Olmsted,
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
1981,
Pubmed
Pesavento,
Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport.
1994,
Pubmed
,
Echinobase
Rashid,
Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP85/95.
1995,
Pubmed
,
Echinobase
Sawin,
Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
1992,
Pubmed
Scholey,
Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.
1986,
Pubmed
,
Echinobase
Scholey,
Isolation of microtubules and a dynein-like MgATPase from unfertilized sea urchin eggs.
1984,
Pubmed
,
Echinobase
Scholey,
Identification of globular mechanochemical heads of kinesin.
1989,
Pubmed
Shakir,
C. elegans osm-3 gene mediating osmotic avoidance behaviour encodes a kinesin-like protein.
1993,
Pubmed
Skoufias,
Cytoplasmic microtubule-based motor proteins.
1993,
Pubmed
Skoufias,
The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding.
1994,
Pubmed
,
Echinobase
Steinhardt,
Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
1994,
Pubmed
,
Echinobase
Tabish,
Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment.
1995,
Pubmed
,
Echinobase
Tyler,
Rotary shadowing of extended molecules dried from glycerol.
1980,
Pubmed
Vale,
One motor, many tails: an expanding repertoire of force-generating enzymes.
1990,
Pubmed
Vale,
Microtubule motors: many new models off the assembly line.
1992,
Pubmed
Vale,
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
1985,
Pubmed
Walker,
Cytoplasmic microtubule-associated motors.
1993,
Pubmed
Walther,
The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein.
1994,
Pubmed
Wedaman,
Sequences of sea urchin kinesin light chain isoforms.
1993,
Pubmed
,
Echinobase
Wilson,
2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans.
1994,
Pubmed
Wright,
Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection.
1993,
Pubmed
,
Echinobase
Wright,
Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
1991,
Pubmed
,
Echinobase
Yamazaki,
KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport.
1995,
Pubmed
Yang,
A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses.
1989,
Pubmed
de Cuevas,
Evidence that the stalk of Drosophila kinesin heavy chain is an alpha-helical coiled coil.
1992,
Pubmed
