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Mol Cell Biol
1996 Mar 01;163:1275-81. doi: 10.1128/MCB.16.3.1275.
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Common factors direct transcription through the proximal sequence elements (PSEs) of the embryonic sea urchin U1, U2, and U6 genes despite minimal similarity among the PSEs.
Li JM
,
Haberman RP
,
Marzluff WF
.
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The proximal sequence element (PSE) for the sea urchin U6 small nuclear RNA gene has been defined. The most critical nucleotides for expression, located 61 to 64 nucleotides (nt) from the transcription start site, are 4 nt, AACT, at the 5'' end of the PSE. Two nucleotide mutations in this region abolish transcription of the sea urchin U6 gene in vitro. The same two nucleotide mutations greatly reduce the binding of specific factors detected by an electrophoretic mobility shift assay. There is also a conserved AC dinucleotide 57 nt from the start site of the sea urchin U1 and U2 PSEs. The sea urchin U1 and U2 PSEs were substituted for the sea urchin U6 PSE, with the conserved AC sequences aligned with those of the U6 PSE. Both of these genes were expressed at levels higher than those observed with the wild-type U6 gene. Similar complexes are formed on the U1 and U2 PSEs, and formation of the complexes is inhibited efficiently by the U6 PSE. In addition, the E-box sequence present upstream of the PSE enhances U6 transcription from both the U1 and U2 PSEs. Finally, depletion of a nuclear extract with a DNA affinity column containing the U6 PSE sequence reduces expression of the U6 genes driven by the U6, U1, or U2 PSE but does not affect expression of the 5S rRNA gene. These data support the possibility that the same factor(s) interacts with the PSE sequences of the U1, U2, and U6 small nuclear RNA genes expressed in early sea urchin embryogenesis.
Bernués,
Common and unique transcription factor requirements of human U1 and U6 snRNA genes.
1993, Pubmed
Bernués,
Common and unique transcription factor requirements of human U1 and U6 snRNA genes.
1993,
Pubmed
De Lorenzi,
Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
1986,
Pubmed
,
Echinobase
Grimm,
The low abundance of U7 snRNA is partly determined by its Sm binding site.
1993,
Pubmed
Henry,
A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III.
1995,
Pubmed
Kadonaga,
Purification of sequence-specific binding proteins by DNA affinity chromatography.
1991,
Pubmed
Kunkel,
U6 small nuclear RNA is transcribed by RNA polymerase III.
1986,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Kunkel,
Transcription of a human U6 small nuclear RNA gene in vivo withstands deletion of intragenic sequences but not of an upstream TATATA box.
1989,
Pubmed
Li,
Transcription of the sea urchin U6 gene in vitro requires a TATA-like box, a proximal sequence element, and sea urchin USF, which binds an essential E box.
1994,
Pubmed
,
Echinobase
Lu,
Cloning and organization of genes for 5S ribosomal RNA in the sea urchin. Lytechinus variegatus.
1981,
Pubmed
,
Echinobase
Lund,
Cyclic 2',3'-phosphates and nontemplated nucleotides at the 3' end of spliceosomal U6 small nuclear RNA's.
1992,
Pubmed
Morris,
Synthesis of U1 RNA in a DNA-dependent system from sea urchin embryos.
1986,
Pubmed
,
Echinobase
Murphy,
Purified octamer binding transcription factors stimulate RNA polymerase III--mediated transcription of the 7SK RNA gene.
1989,
Pubmed
Murphy,
Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes.
1992,
Pubmed
Nash,
A developmental switch in sea urchin U1 RNA.
1989,
Pubmed
,
Echinobase
Nijhawan,
Metabolism of low molecular weight ribonucleic acids in early sea urchin embryos.
1979,
Pubmed
,
Echinobase
Phillips,
A transcriptional analysis of the gene encoding mouse U7 small nuclear RNA.
1992,
Pubmed
Reddy,
The capped U6 small nuclear RNA is transcribed by RNA polymerase III.
1987,
Pubmed
Sadowski,
Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE.
1993,
Pubmed
Sakallah,
Isolation and characterization of the tandemly repeated U6 genes from the sea urchin Strongylocentrotus purpuratus.
1994,
Pubmed
,
Echinobase
Santiago,
Expression of the U1 RNA gene repeat during early sea urchin development: evidence for a switch in U1 RNA genes during development.
1989,
Pubmed
,
Echinobase
Southgate,
In vivo and in vitro expression of U7 snRNA genes: cis- and trans-acting elements required for RNA polymerase II-directed transcription.
1989,
Pubmed
,
Echinobase
Stefanovic,
The proximal element is capable of determining proper temporal expression of the embryonic sea urchin U2 snRNA gene.
1994,
Pubmed
,
Echinobase
Stefanovic,
Isolation and characterization of developmentally regulated sea urchin U2 snRNA genes.
1991,
Pubmed
,
Echinobase
Stefanovic,
Characterization of two developmentally regulated sea urchin U2 small nuclear RNA promoters: a common required TATA sequence and independent proximal and distal elements.
1992,
Pubmed
,
Echinobase
Tanaka,
Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.
1992,
Pubmed
Waldschmidt,
Identification of transcription factors required for the expression of mammalian U6 genes in vitro.
1991,
Pubmed
Wendelburg,
Two promoter elements are necessary and sufficient for expression of the sea urchin U1 snRNA gene.
1992,
Pubmed
,
Echinobase
Yoon,
Proximal sequence element-binding transcription factor (PTF) is a multisubunit complex required for transcription of both RNA polymerase II- and RNA polymerase III-dependent small nuclear RNA genes.
1995,
Pubmed
Yoon,
Cloning of two proximal sequence element-binding transcription factor subunits (gamma and delta) that are required for transcription of small nuclear RNA genes by RNA polymerases II and III and interact with the TATA-binding protein.
1996,
Pubmed
