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J Cell Biol
1995 Dec 01;1315:1183-92. doi: 10.1083/jcb.131.5.1183.
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Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.
Whalley T
,
Terasaki M
,
Cho MS
,
Vogel SS
.
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At fertilization in sea urchin eggs, elevated cytosolic Ca2+ leads to the exocytosis of 15,000-18,000 1.3-microns-diam cortical secretory granules to form the fertilization envelope. Cortical granule exocytosis more than doubles the surface area of the egg. It is thought that much of the added membrane is retrieved by subsequent endocytosis. We have investigated how this is achieved by activating eggs in the presence of aqueous- and lipid-phase fluorescent dyes. We find rapid endocytosis of membrane into 1.5-microns-diam vesicles starting immediately after cortical granule exocytosis and persisting over the following 15 min. The magnitude of this membrane retrieval can compensate for the changes in the plasma membrane of the egg caused by exocytosis. This membrane retrieval is not stimulated by PMA treatment which activates the endocytosis of clathrin-coated vesicles. When eggs are treated with short wave-length ultraviolet light, cortical granule exocytosis still occurs, but granule cores fail to disperse. After egg activation, large vesicles containing semi-intact cortical granule protein cores are observed. These data together with experiments using sequential pulses of fluid-phase markers support the hypothesis that the bulk of membrane retrieval immediately after cortical granule exocytosis is achieved through direct retrieval into large endocytotic structures.
Alliegro,
Storage and mobilization of extracellular matrix proteins during sea urchin development.
1988, Pubmed,
Echinobase
Alliegro,
Storage and mobilization of extracellular matrix proteins during sea urchin development.
1988,
Pubmed
,
Echinobase
Alvarez de Toledo,
The events leading to secretory granule fusion.
1988,
Pubmed
Alvarez de Toledo,
Release of secretory products during transient vesicle fusion.
1993,
Pubmed
Baker,
Secretion-dependent uptake of extracellular fluid by the rat neurohypophysis.
1974,
Pubmed
Baker,
Calcium control of exocytosis and endocytosis in bovine adrenal medullary cells.
1981,
Pubmed
Betz,
Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction.
1992,
Pubmed
Bisgrove,
Fibropellins, products of an EGF repeat-containing gene, form a unique extracellular matrix structure that surrounds the sea urchin embryo.
1991,
Pubmed
,
Echinobase
Breckenridge,
Currents through the fusion pore that forms during exocytosis of a secretory vesicle.
1987,
Pubmed
Brown,
Recycling receptors: the round-trip itinerary of migrant membrane proteins.
1983,
Pubmed
Chandler,
High molecular weight polymers block cortical granule exocytosis in sea urchin eggs at the level of granule matrix disassembly.
1989,
Pubmed
,
Echinobase
Chandler,
Multiple intracellular signals coordinate structural dynamics in the sea urchin egg cortex at fertilization.
1991,
Pubmed
,
Echinobase
Chen,
Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosis.
1991,
Pubmed
Chernomordik,
Lysolipids reversibly inhibit Ca(2+)-, GTP- and pH-dependent fusion of biological membranes.
1993,
Pubmed
,
Echinobase
Chernomordik,
Biomembrane fusion: a new concept derived from model studies using two interacting planar lipid bilayers.
1987,
Pubmed
Ciapa,
Structural modifications induced by TPA (12-O-tetradecanoyl phorbol-13-acetate) in sea urchin eggs.
1988,
Pubmed
,
Echinobase
Crossley,
Guanosine 5'-thiotriphosphate may stimulate phosphoinositide messenger production in sea urchin eggs by a different route than the fertilizing sperm.
1991,
Pubmed
,
Echinobase
Curran,
Exocytotic fusion pores exhibit semi-stable states.
1993,
Pubmed
Deits,
Purification and properties of ovoperoxidase, the enzyme responsible for hardening the fertilization membrane of the sea urchin egg.
1984,
Pubmed
,
Echinobase
Faire,
Sea urchin egg 100-kDa dynamin-related protein: identification of and localization to intracellular vesicles.
1993,
Pubmed
,
Echinobase
Fernandez,
Capacitance measurements reveal stepwise fusion events in degranulating mast cells.
1985,
Pubmed
Fisher,
Sea urchin egg cortical granule exocytosis is followed by a burst of membrane retrieval via uptake into coated vesicles.
1983,
Pubmed
,
Echinobase
Fisher,
Analysis of sea urchin egg cortical transformation in the absence of cortical granule exocytosis.
1985,
Pubmed
,
Echinobase
Foltz,
Sea urchin egg receptor for sperm: sequence similarity of binding domain and hsp70.
1993,
Pubmed
,
Echinobase
Hall,
Hardening of the sea urchin fertilization envelope by peroxidase-catalyzed phenolic coupling of tyrosines.
1978,
Pubmed
,
Echinobase
Heuser,
Structural changes after transmitter release at the frog neuromuscular junction.
1981,
Pubmed
Hylander,
The effect of local anesthetics and ammonia on cortical granule-plasma membrane attachment in the sea urchin egg.
1981,
Pubmed
,
Echinobase
Jaffe,
The time course of cortical vesicle fusion in sea urchin eggs observed as membrane capacitance changes.
1978,
Pubmed
,
Echinobase
Kay,
Ovoperoxidase assembly into the sea urchin fertilization envelope and dityrosine crosslinking.
1987,
Pubmed
,
Echinobase
Kirchhausen,
Protein organization in clathrin trimers.
1981,
Pubmed
Kline,
The timing of cortical granule fusion, content dispersal, and endocytosis during fertilization of the hamster egg: an electrophysiological and histochemical study.
1994,
Pubmed
Lane,
A role for regulated secretion of apical extracellular matrix during epithelial invagination in the sea urchin.
1993,
Pubmed
,
Echinobase
Lee,
Changes in intracellular acidic compartments in sea urchin eggs after activation.
1983,
Pubmed
,
Echinobase
Malencik,
Dityrosine formation in calmodulin.
1987,
Pubmed
Markin,
On the theory of membrane fusion. The stalk mechanism.
1984,
Pubmed
Mohri,
Quantitative analysis of the process and propagation of cortical granule breakdown in sea urchin eggs.
1990,
Pubmed
,
Echinobase
Monck,
The exocytotic fusion pore.
1992,
Pubmed
Monck,
Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore.
1990,
Pubmed
Nagasawa,
Micropinocytotic origin of coated and smooth microvesicles ("synaptic vesicles") in neurosecretory terminals of posterior pituitary glands demonstrated by incorporation of horseradish peroxidase.
1971,
Pubmed
Nordmann,
Membrane retrieval following exocytosis in isolated neurosecretory nerve endings.
1992,
Pubmed
Obar,
Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins.
1990,
Pubmed
Rosenboom,
Exo-endocytosis and closing of the fission pore during endocytosis in single pituitary nerve terminals internally perfused with high calcium concentrations.
1994,
Pubmed
Rothman,
A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting.
1990,
Pubmed
Ryan,
The kinetics of synaptic vesicle recycling measured at single presynaptic boutons.
1993,
Pubmed
Sardet,
The ultrastructure of the sea urchin egg cortex isolated before and after fertilization.
1984,
Pubmed
,
Echinobase
Schroeder,
Surface area change at fertilization: resorption of the mosaic membrane.
1979,
Pubmed
,
Echinobase
Smythe,
The mechanism of receptor-mediated endocytosis.
1991,
Pubmed
Swann,
The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs.
1986,
Pubmed
,
Echinobase
Terasaki,
Visualization of exocytosis during sea urchin egg fertilization using confocal microscopy.
1995,
Pubmed
,
Echinobase
Thilo,
Selective internalization of granule membrane after secretion in mast cells.
1985,
Pubmed
Thomas,
A triggered mechanism retrieves membrane in seconds after Ca(2+)-stimulated exocytosis in single pituitary cells.
1994,
Pubmed
Ungewickell,
Assembly units of clathrin coats.
1981,
Pubmed
Vogel,
The sea urchin cortical reaction. A model system for studying the final steps of calcium-triggered vesicle fusion.
1991,
Pubmed
,
Echinobase
Vogel,
Lysophosphatidylcholine reversibly arrests exocytosis and viral fusion at a stage between triggering and membrane merger.
1993,
Pubmed
,
Echinobase
Watts,
Endocytosis: what goes in and how?
1992,
Pubmed
Zimmerberg,
Mechanisms of membrane fusion.
1993,
Pubmed
Zimmerberg,
Simultaneous electrical and optical measurements show that membrane fusion precedes secretory granule swelling during exocytosis of beige mouse mast cells.
1987,
Pubmed
von Gersdorff,
Dynamics of synaptic vesicle fusion and membrane retrieval in synaptic terminals.
1994,
Pubmed
