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J Cell Biol
1993 Nov 01;1233:681-9. doi: 10.1083/jcb.123.3.681.
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Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection.
Wright BD
,
Terasaki M
,
Scholey JM
.
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Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833). Here, we report that the microinjection of KHC-specific antibodies into these cells has no effect on mitosis or ER membrane organization, even though one such antibody, SUK4, blocks kinesin-driven motility in vitro and in mammalian cells. Microinjected SUK4 was localized to early mitotic figures, suggesting that it is able to access kinesin in spindles. In contrast to KHC-specific antibodies, two antibodies that react with kinesin-like proteins (KLPs), namely CHO1 and HD, disrupted mitosis and prevented subsequent cell division. CHO1 is thought to exert this effect by blocking the activity of a 110-kD KLP. The relevant target of HD, which was raised against the KHC motor domain, is unknown; HD may disrupt mitosis by interfering with an essential spindle KLP but not with KHC itself, as preabsorption of HD with KHC did not alter its ability to block mitosis. These data indicate that some KLPs have essential mitotic functions in early sea urchin embryos but KHC itself does not.
Bukovsky,
Simple and rapid purification of monoclonal antibodies from cell culture supernatants and ascites fluids by hydroxylapatite chromatography on analytical and preparative scales.
1987, Pubmed
Bukovsky,
Simple and rapid purification of monoclonal antibodies from cell culture supernatants and ascites fluids by hydroxylapatite chromatography on analytical and preparative scales.
1987,
Pubmed
Cole,
Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
1992,
Pubmed
,
Echinobase
Hall,
Kinesin-related gene unc-104 is required for axonal transport of synaptic vesicles in C. elegans.
1991,
Pubmed
Henson,
Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
1992,
Pubmed
,
Echinobase
Henson,
A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo.
1989,
Pubmed
,
Echinobase
Hinkley,
Rapid visual detection of sperm-egg fusion using the DNA-specific fluorochrome Hoechst 33342.
1986,
Pubmed
,
Echinobase
Hiramoto,
Micromanipulation studies of the mitotic apparatus in sand dollar eggs.
1988,
Pubmed
,
Echinobase
Hiramoto,
Micromanipulation.
1984,
Pubmed
Hollenbeck,
Radial extension of macrophage tubular lysosomes supported by kinesin.
1990,
Pubmed
Ingold,
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
1988,
Pubmed
,
Echinobase
Kiehart,
Microinjection of echinoderm eggs: apparatus and procedures.
1982,
Pubmed
,
Echinobase
McIntosh,
Mitotic motors.
1991,
Pubmed
Nislow,
A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles.
1992,
Pubmed
Nislow,
A monoclonal antibody to a mitotic microtubule-associated protein blocks mitotic progression.
1990,
Pubmed
Oberdorf,
Identification of a calsequestrin-like protein from sea urchin eggs.
1988,
Pubmed
,
Echinobase
Rappaport,
Establishment of the mechanism of cytokinesis in animal cells.
1986,
Pubmed
Rebhun,
In vitro reactivation of anaphase B in isolated spindles of the sea urchin egg.
1988,
Pubmed
,
Echinobase
Rodionov,
Kinesin is responsible for centrifugal movement of pigment granules in melanophores.
1991,
Pubmed
Sawin,
Motor proteins in cell division.
1991,
Pubmed
Saxton,
Kinesin heavy chain is essential for viability and neuromuscular functions in Drosophila, but mutants show no defects in mitosis.
1991,
Pubmed
Scholey,
Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.
1986,
Pubmed
,
Echinobase
Sellitto,
Distribution of a matrix component of the midbody during the cell cycle in Chinese hamster ovary cells.
1988,
Pubmed
Sluder,
The role of spindle microtubules in the timing of the cell cycle in echinoderm eggs.
1986,
Pubmed
,
Echinobase
Terasaki,
Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.
1991,
Pubmed
,
Echinobase
Vallee,
Potential roles of microtubule-associated motor molecules in cell division.
1990,
Pubmed
Wright,
Microtubule motors in the early sea urchin embryo.
1992,
Pubmed
,
Echinobase
Wright,
Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
1991,
Pubmed
,
Echinobase
