ECB-ART-35907
J Cell Biol
1994 Nov 01;1274:973-84. doi: 10.1083/jcb.127.4.973.
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Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos.
Hamill D
,
Davis J
,
Drawbridge J
,
Suprenant KA
.
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A subset of mRNAs, polyribosomes, and poly(A)-binding proteins copurify with microtubules from sea urchin embryos. Several lines of evidence indicate that the interaction of microtubules with ribosomes is specific: a distinct stalk-like structure appears to mediate their association; ribosomes bind to microtubules with a constant stoichiometry through several purification cycles; and the presence of ribosomes in these preparations depends on the presence of intact microtubules. Five specific mRNAs are enriched with the microtubule-bound ribosomes, indicating that translation of specific proteins may occur on the microtubule scaffolding in vivo.
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Genes referenced: LOC100887844 LOC100893907
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