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ECB-ART-35553
Biochim Biophys Acta 1980 Oct 17;6093:456-63. doi: 10.1016/0005-2787(80)90119-7.
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A potential DNA replication complex isolated from sea urchin embryos by renografin gradient centrifugation.

Crabb WD , Firshein W , Infante AA .


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A complex containing deoxyribonucleic acid, protein and lipid has been isolated from the nuclei of the sea urchin Stronglocentrotus pupuratus by gentle lysis of nuclei and centrifugation of the lysate in a renografin gradient. The complex is similar in several respects to DNA-membrane complexes isolated by the sarkosyl M-band technique [1]. These include the following: 1, most of the cellular DNA is present in the complex but the bulk of the DNA may be removed by sonication or nuclease treatment; 2, nascent DNA is preferentially found in the complex; and 3, destruction of membrane/protein components removes DNA found in the complex.

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Genes referenced: LOC100887844