Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1981 Jul 01;787:4078-82. doi: 10.1073/pnas.78.7.4078.
Show Gene links
Show Anatomy links
Isolation of two clusters of mouse histone genes.
Sittman DB
,
Chiu IM
,
Pan CJ
,
Cohn RH
,
Kedes LH
,
Marzluff WF
.
???displayArticle.abstract???
Histone mRNA was partially purified from mouse myeloma cells synchronized in S phase by isoleucine starvation. A cDNA was prepared that contained sequences complementary to all five mouse histone genes. This cDNA was used to screen a library of mouse DNA in lambda phage. The positive clones were screened by hybridization with sea urchin histone gene-specific probes to identify those clones that contained histone genes. Confirmation of this identification was obtained by hybridization with Drosophila histone genes. Two independent clusters of histone genes were isolated. One, MM531, contains regions hybridizing specifically to H3, H4, and H1 and the other, MM221, contains two regions hybridizing specifically to H3 and single regions complementary to H4, H2b, and H2a. They are not part of a simple repeating structure. The nucleotide sequence of the coding region of the H3 gene in MM531 has been determined. This gene could code for a variant H3 protein that has several amino acid substitutions not reported in other H3 proteins.
Anderson,
Changing rates of histone mRNA synthesis and turnover in Drosophila embryos.
1980, Pubmed
Anderson,
Changing rates of histone mRNA synthesis and turnover in Drosophila embryos.
1980,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Blattner,
Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
1977,
Pubmed
Blattner,
Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
1978,
Pubmed
Borun,
Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger.
1967,
Pubmed
Borun,
Further evidence of transcriptional and translational control of histone messenger RNA during the HeLa S3 cycle.
1975,
Pubmed
Breindl,
On the translational control of histone synthesis. Quantitation of biologically active histone mRNA from synchronized HeLa cells and its translation in different cell-free systems.
1974,
Pubmed
Childs,
Orphons: dispersed genetic elements derived from tandem repetitive genes of eucaryotes.
1981,
Pubmed
,
Echinobase
Cohen,
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
1972,
Pubmed
Cohn,
Histone genes of the sea urchin (S. purpuratus) cloned in E coli: order, polarity, and strandedness of the five histone-coding and spacer regions.
1976,
Pubmed
,
Echinobase
Crawford,
Histone genes are clustered with a 15-kilobase repeat in the chicken genome.
1979,
Pubmed
Franklin,
Non-allelic variants of histones 2a, 2b and 3 in mammals.
1977,
Pubmed
Gross,
Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.
1976,
Pubmed
,
Echinobase
Hereford,
Isolation of yeast histone genes H2A and H2B.
1979,
Pubmed
Hieter,
Histone gene switch in the sea urchin embryo. Identification of late embryonic histone messenger ribonucleic acids and the control of their synthesis.
1979,
Pubmed
,
Echinobase
Isenberg,
Histones.
1979,
Pubmed
Jacob,
Histone-gene reiteration in the genome of mouse.
1976,
Pubmed
,
Echinobase
Kedes,
Histone genes and histone messengers.
1979,
Pubmed
Kinkade,
The resolution of four lysine-rich histones derived from calf thymus.
1966,
Pubmed
Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed
Lichtler,
Isolation and characterization of two mRNAs from HeLa S3 cells coding for histone H4.
1977,
Pubmed
Lifton,
The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications.
1978,
Pubmed
,
Echinobase
Maniatis,
The isolation of structural genes from libraries of eucaryotic DNA.
1978,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Newrock,
Stage-specific mRNAs coding for subtypes of H2A and H2B histones in the sea urchin embryo.
1978,
Pubmed
,
Echinobase
Panyim,
High resolution acrylamide gel electrophoresis of histones.
1969,
Pubmed
Sippel,
Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
1973,
Pubmed
Smith,
In vivo effects of intercalating drugs on the superhelix density of mitochondrial DNA isolated from human and mouse cells in culture.
1971,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Stein,
Regulation of cell cycle stage-specific transcription of histone genes from chromatin by non-histone chromosomal proteins.
1975,
Pubmed
Sures,
The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions.
1978,
Pubmed
,
Echinobase
Taylor,
Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase.
1976,
Pubmed
Vogelstein,
Preparative and analytical purification of DNA from agarose.
1979,
Pubmed
Wallis,
Histone H2B genes of yeast encode two different proteins.
1980,
Pubmed
Zweidler,
Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents.
1978,
Pubmed
