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J Cell Biol
1972 May 01;532:474-82. doi: 10.1083/jcb.53.2.474.
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Stabilities of nuclear and messenger RNA molecules in sea urchin embryos.
Brandhorst BP
,
Humphreys T
.
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The kinetics of accumulation of radioactive adenosine in adenosine triphosphate and in RNA of nuclear, cytoplasmic, and polysomal fractions of sea urchin embryos have been analyzed. 85% of the RNA synthesized decays in the nucleus with an apparently uniform half-life of about 7 min. The remaining 15% goes to the cytoplasm, mostly entering polysomes, and decays with a quite uniform half-life of about 75 min. The nuclear RNA accounts for one-third and the cytoplasmic RNA accounts for two-thirds of the total unstable RNA which accumulates at steady state in the embryo. The size distribution of short-labeled nuclear RNA is very similar to that of long-labeled messenger RNA, when both are extracted directly from the cells without a previous cell fractionation.
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Properties of nuclear RNA in sea urchin embryos.
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Properties of nuclear RNA in sea urchin embryos.
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Synthesis and decay rates of major classes of deoxyribonucleic acid a5brandhorst BP, Humphreys T: Synthesis and decay rates of major classes of deoxyribonucleic acid-like ribonucleic acid in sea urchin embryos.
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,
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,
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,
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The isolation of nuclei from eggs and embryos of the sea urchin.
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,
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Synthesis and function of messenger RNA during early embryonic development.
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Distribution of mRNA in the cytoplasmic polyribosomes of the HeLa cell.
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Lindberg,
SV40-specific RNA in the nucleus and polyribosomes of transformed cells.
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PENMAN,
A CYTOPLASMIC STRUCTURE INVOLVED IN THE SYNTHESIS AND ASSEMBLY OF POLIOVIRUS COMPONENTS.
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Penman,
POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA.
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Penman,
Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells.
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Scherrer,
Patterns of RNA metabolism in a differentiated cell: a rapidly labeled, unstable 60S RNA with messenger properties in duck erythroblasts.
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Soeiro,
The turnover of nuclear DNA-like RNA in HeLa cells.
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Thaler,
Isolation of nuclei from sea urchin eggs and embryos.
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,
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An artifact in the alkaline hydrolysis of RNA labeled with guanosine-8-T.
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