Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1985 Jun 01;1006:1817-24. doi: 10.1083/jcb.100.6.1817.
Show Gene links
Show Anatomy links
Involvement of zinc in the regulation of pHi, motility, and acrosome reactions in sea urchin sperm.
Clapper DL
,
Davis JA
,
Lamothe PJ
,
Patton C
,
Epel D
.
???displayArticle.abstract???
When sperm of Strongylocentrotus purpuratus or Lytechinus pictus are diluted into seawater, motility is initiated; and when exposed to egg jelly, an acrosome reaction is induced. In the presence of a variety of structurally different metal chelators (0.1-1 mM EDTA, EGTA, phenanthroline, dipyridyl, cysteine, or dithiothreitol), motility initiation is delayed and the acrosome reaction is inhibited. Of the metals detected in the sperm of these two species, very low levels of Zn+2 (0.1 microM free Zn+2) uniquely prevent this chelator inhibition. L. pictus sperm concentrate 65Zn+2 from seawater, and EDTA removes 50% of the accumulated 65Zn+2 by 5 min. Since both sperm motility and acrosome reactions are in part regulated by intracellular pH (pHi), the effect of chelators on the sperm pHi was examined by using the fluorescent pH sensitive probe, 9-aminoacridine, EDTA depresses sperm pHi in both species, and 0.1 microM free Zn+2 reverses this pHi depression. When sperm are diluted into media that contain chelators, both NH4Cl and monensin (a Na+/H+ ionophore) increase the sperm pHi and reverse the chelator inhibition of sperm motility and acrosome reactions. The results of this study are consistent with the involvement of a trace metal (probably zinc) in the pHi regulation of sea urchin sperm and indicate a likely mechanism for the previously observed effects of chelators on sperm motility and acrosome reactions.
Alitalo,
Effects of Zn2+ ions on protein phosphorylation in epithelial cell membranes.
1983, Pubmed
Alitalo,
Effects of Zn2+ ions on protein phosphorylation in epithelial cell membranes.
1983,
Pubmed
Christen,
Elevation of the intracellular pH activates respiration and motility of sperm of the sea urchin, Strongylocentrotus purpuratus.
1982,
Pubmed
,
Echinobase
Delgado,
Modification on human sperm metabolism by the induced release of intracellular zinc.
1975,
Pubmed
Johnson,
A volatile inhibitor immobilizes sea urchin sperm in semen by depressing the intracellular pH.
1983,
Pubmed
,
Echinobase
Johnson,
Heavy metal chelators prolong motility and viability of sea urchin sperm by inhibiting spontaneous acrosome reactions.
1983,
Pubmed
,
Echinobase
Kopf,
Correlation between sea urchin sperm respiratory rates and cyclic AMP concentrations as a function of cell dilution.
1978,
Pubmed
,
Echinobase
Lee,
Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm.
1983,
Pubmed
,
Echinobase
Lindholmer,
Toxicity of zinc ions to human spermatozoa and the influence of albumin.
1974,
Pubmed
Meizel,
Evidence for the role of a trypsin-like enzyme in the hamster sperm acrosome reaction.
1976,
Pubmed
Morisawa,
Heavy metals and spermatozoan motility. III conformational changes induced by divalent cations and by ATP in flagellar proteins.
1976,
Pubmed
,
Echinobase
Morisawa,
Heavy metals and spermatozoan motility. I. Distribution of iron, zinc and copper in sea urchin spermatozoa.
1972,
Pubmed
,
Echinobase
Saito,
Effects of certain metals and chelating agents on rat and dog epididymal spermatozoan motility.
1967,
Pubmed
Santos-Sacchi,
Potentiation of the cGMP-induced guinea pig acrosome reaction by zinc.
1980,
Pubmed
Schackmann,
A partial sequence of ionic changes associated with the acrosome reaction of Strongylocentrotus purpuratus.
1981,
Pubmed
,
Echinobase
Schackmann,
Membrane potential depolarization and increased intracellular pH accompany the acrosome reaction of sea urchin sperm.
1981,
Pubmed
,
Echinobase
Schackmann,
The acrosome reaction of Strongylocentrotus purpuratus sperm. Ion requirements and movements.
1978,
Pubmed
,
Echinobase
Swarup,
Phosphoprotein phosphatase activity of sea urchin spermatozoa.
1982,
Pubmed
,
Echinobase
WHITE,
The toxicity of heavy metals to mammalian spermatozoa.
1955,
Pubmed
Ward,
Dephosphorylation of a major sperm membrane protein is induced by egg jelly during sea urchin fertilization.
1983,
Pubmed
,
Echinobase
Young,
The effects of heavy metal ions on the motility of sea urchin spermatozoa.
1974,
Pubmed
,
Echinobase
