Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Physiol
1988 Feb 01;396:189-204. doi: 10.1113/jphysiol.1988.sp016958.
Show Gene links
Show Anatomy links
Cations that alter surface potentials of lipid bilayers increase the calcium requirement for exocytosis in sea urchin eggs.
McLaughlin S
,
Whitaker M
.
???displayArticle.abstract???
1. We examined the effects of cations that bind to phospholipid bilayers on the calcium requirement for exocytosis in vitro. 2. Tetracaine and trifluoperazine, two monovalent cations that bind hydrophobically to lipid bilayers, decreased the zeta-potential of lipid vesicles with a phospholipid composition similar to sea urchin plasma membranes; the decrease in the magnitude of the negative zeta-potential was consistent with the Gouy-Chapman-Stern theory. 3. Trifluoperazine and tetracaine also increased the concentration of calcium required to produce half-maximal exocytosis (Ca50) in isolated fragments of plasma membranes from sea urchin eggs. 4. The effects of trifluoperazine and tetracaine on the Ca50 of egg fragments can be explained quantitatively from the zeta-potential data obtained with phospholipid vesicles if we assume the calcium binding sites responsible for triggering exocytosis lie within a Debye length (less than 1 nm) of the surface of the plasma membranes, such that the concentration of calcium at the sites is affected by the surface potential of the membrane. 5. The divalent cation magnesium, which binds specifically to the phosphate group of lipids, affected Ca50 in a manner that can also be explained quantitatively from its effects on the zeta-potential of phospholipid vesicles. 6. The polyvalent cation neomycin, which adsorbs to the lipid phosphatidylinositol 4,5-bisphosphate with high affinity, also binds co-operatively to phosphatidylinositol; its effect on Ca50 can be explained quantitatively from its effects on the zeta-potential of vesicles containing the latter lipid. 7. A decrease in the ionic strength increases the magnitude of the surface potential of membranes; the effect of ionic strength on Ca50 can be explained qualitatively from its effect on the zeta-potential of phospholipid vesicles. 8. All our results are consistent with the hypothesis that calcium binding sites lie within a Debye length of the plasma membrane; they also indicate that drugs with specific, high-affinity sites may affect exocytosis by a non-specific mechanism.
Alvarez,
Large divalent cations and electrostatic potentials adjacent to membranes. Experimental results with hexamethonium.
1983, Pubmed
Alvarez,
Large divalent cations and electrostatic potentials adjacent to membranes. Experimental results with hexamethonium.
1983,
Pubmed
Anderson,
Regulation of the association of membrane skeletal protein 4.1 with glycophorin by a polyphosphoinositide.
1985,
Pubmed
Baker,
Influence of ATP and calcium on the cortical reaction in sea urchin eggs.
1978,
Pubmed
,
Echinobase
Chung,
Interaction of gentamicin and spermine with bilayer membranes containing negatively charged phospholipids.
1985,
Pubmed
Cockcroft,
Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase.
1985,
Pubmed
Crabb,
In vitro reconstitution of exocytosis from plasma membrane and isolated secretory vesicles.
1985,
Pubmed
,
Echinobase
Eisenberg,
Adsorption of monovalent cations to bilayer membranes containing negative phospholipids.
1979,
Pubmed
Feigenson,
On the nature of calcium ion binding between phosphatidylserine lamellae.
1986,
Pubmed
Holz,
Effects of osmolality and ionic strength on secretion from adrenal chromaffin cells permeabilized with digitonin.
1986,
Pubmed
Illingworth,
A common source of error in pH measurements.
1981,
Pubmed
Josepovitz,
Inhibition of gentamicin uptake in rat renal cortex in vivo by aminoglycosides and organic polycations.
1982,
Pubmed
Kamel,
Phosphatidylinositol metabolism during fertilization in the sea urchin egg.
1985,
Pubmed
,
Echinobase
Lau,
The adsorption of divalent cations to phosphatidylglycerol bilayer membranes.
1981,
Pubmed
Loosley-Millman,
Effects of monovalent ion binding and screening on measured electrostatic forces between charged phospholipid bilayers.
1982,
Pubmed
Marra,
Direct measurement of the interaction between phosphatidylglycerol bilayers in aqueous electrolyte solutions.
1986,
Pubmed
McLaughlin,
The hydrophobic adsorption of charged molecules to bilayer membranes: a test of the applicability of the stern equation.
1976,
Pubmed
McLaughlin,
Adsorption of divalent cations to bilayer membranes containing phosphatidylserine.
1981,
Pubmed
Roth,
All lipid-soluble anaesthetics protect red cells.
1971,
Pubmed
Sanchez,
Trifluoperazine and chlorpromazine block secretion from human platelets evoked at basal cytoplasmic free calcium by activators of C-kinase.
1983,
Pubmed
Sasaki,
Cortical vesicle exocytosis in isolated cortices of sea urchin eggs: description of a turbidometric assay and its utilization in studying effects of different media on discharge.
1983,
Pubmed
,
Echinobase
Schacht,
Inhibition by neomycin of polyphosphoinositide turnover in subcellular fractions of guinea-pig cerebral cortex in vitro.
1976,
Pubmed
Schibeci,
Action of neomycin on the metabolism of polyphosphoinositides in the guinea pig kidney.
1977,
Pubmed
Schmell,
Phospholipid metabolism in the eggs and embryos of the sea urchin Arbacia punctulata.
1974,
Pubmed
,
Echinobase
Turner,
Fertilization increases the polyphosphoinositide content of sea urchin eggs.
1984,
Pubmed
,
Echinobase
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Weiss,
Mechanism for selectively inhibiting the activation of cyclic nucleotide phosphodiesterase and adenylate cyclase by antipsychotic agents.
1978,
Pubmed
Whitaker,
Calcium-dependent exocytosis in an in vitro secretory granule plasma membrane preparation from sea urchin eggs and the effects of some inhibitors of cytoskeletal function.
1983,
Pubmed
,
Echinobase
Whitaker,
Calcium-dependent polyphosphoinositide hydrolysis is associated with exocytosis in vitro.
1985,
Pubmed
,
Echinobase
Zimmerberg,
Exocytosis of sea urchin egg cortical vesicles in vitro is retarded by hyperosmotic sucrose: kinetics of fusion monitored by quantitative light-scattering microscopy.
1985,
Pubmed
,
Echinobase
