Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1988 Dec 01;1076 Pt 2:2657-67. doi: 10.1083/jcb.107.6.2657.
Show Gene links
Show Anatomy links
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
Ingold AL
,
Cohn SA
,
Scholey JM
.
???displayArticle.abstract???
We have prepared and characterized seven mouse monoclonal antibodies (SUK 1-7) to the 130-kD heavy chain of sea urchin egg kinesin. On immunoblots, SUK 3 and SUK 4 cross-reacted with Drosophila embryo 116-kD heavy chains, and SUK 4, SUK 5, SUK 6, and SUK 7 bound to the 120-kD heavy chains of bovine brain kinesin. Three out of seven monoclonal antikinesins (SUK 4, SUK 6, and SUK 7) caused a dose-dependent inhibition of sea urchin egg kinesin-induced microtubule translocation, whereas the other four monoclonal antibodies had no detectable effect on this motility. The inhibitory monoclonal antibodies (SUK 4, SUK 6, and SUK 7) appear to bind to spatially related sites on an ATP-sensitive microtubule binding 45-kD chymotryptic fragment of the 130-kD heavy chain, whereas SUK 2 binds to a spatially distinct site. None of the monoclonal antikinesins inhibited the microtubule activated MgATPase activity of kinesin, suggesting that SUK 4, SUK 6, and SUK 7 uncouple this MgATPase activity from motility.
Adams,
Propulsion of organelles isolated from Acanthamoeba along actin filaments by myosin-I.
1986, Pubmed
Adams,
Propulsion of organelles isolated from Acanthamoeba along actin filaments by myosin-I.
1986,
Pubmed
Amos,
Kinesin from pig brain studied by electron microscopy.
1987,
Pubmed
Bloom,
Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
1988,
Pubmed
Brady,
A novel brain ATPase with properties expected for the fast axonal transport motor.
1985,
Pubmed
Citi,
Studies on the structure and conformation of brush border myosin using monoclonal antibodies.
1987,
Pubmed
Cohn,
Correlation between the ATPase and microtubule translocating activities of sea urchin egg kinesin.
1987,
Pubmed
,
Echinobase
Flicker,
Site-specific inhibition of myosin-mediated motility in vitro by monoclonal antibodies.
1985,
Pubmed
Gelles,
Tracking kinesin-driven movements with nanometre-scale precision.
1988,
Pubmed
Gibbons,
The effect of antidynein 1 serum on the movement of reactivated sea urchin sperm.
1976,
Pubmed
,
Echinobase
Kappler,
A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product.
1987,
Pubmed
Kiehart,
Antibody inhibitors of nonmuscle myosin function and assembly.
1986,
Pubmed
Kiehart,
Evidence that myosin does not contribute to force production in chromosome movement.
1982,
Pubmed
,
Echinobase
Kiehart,
Monoclonal antibodies demonstrate limited structural homology between myosin isozymes from Acanthamoeba.
1984,
Pubmed
Kiehart,
Direct localization of monoclonal antibody-binding sites on Acanthamoeba myosin-II and inhibition of filament formation by antibodies that bind to specific sites on the myosin-II tail.
1984,
Pubmed
Kiehart,
Inhibition of acanthamoeba actomyosin-II ATPase activity and mechanochemical function by specific monoclonal antibodies.
1984,
Pubmed
Kuznetsov,
Bovine brain kinesin is a microtubule-activated ATPase.
1986,
Pubmed
Kuznetsov,
The quaternary structure of bovine brain kinesin.
1988,
Pubmed
Leslie,
Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.
1987,
Pubmed
,
Echinobase
Mabuchi,
The effect of myosin antibody on the division of starfish blastomeres.
1977,
Pubmed
,
Echinobase
Markwell,
Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril.
1978,
Pubmed
Neighbors,
Localization of kinesin in cultured cells.
1988,
Pubmed
Ogawa,
Preparation of antiserum against a tryptic fragment (fragment A) of dynein and an immunological approach to the subunit composition of dynein.
1975,
Pubmed
,
Echinobase
Okuno,
Inhibition of movement and ATP-ase activity of demembranated sea urchin spermatozoa by anti-dynein antiserum.
1976,
Pubmed
,
Echinobase
Peltz,
Monoclonal antibodies against seven sites on the head and tail of Dictyostelium myosin.
1985,
Pubmed
Porter,
Characterization of the microtubule movement produced by sea urchin egg kinesin.
1987,
Pubmed
,
Echinobase
Porter,
Dynein isoforms in sea urchin eggs.
1988,
Pubmed
,
Echinobase
Pryer,
Polarized microtubule gliding and particle saltations produced by soluble factors from sea urchin eggs and embryos.
1986,
Pubmed
,
Echinobase
Saxton,
Drosophila kinesin: characterization of microtubule motility and ATPase.
1988,
Pubmed
,
Echinobase
Scholey,
Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.
1986,
Pubmed
,
Echinobase
Scholey,
Isolation of microtubules and a dynein-like MgATPase from unfertilized sea urchin eggs.
1984,
Pubmed
,
Echinobase
Vale,
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
1985,
Pubmed
Vale,
Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro.
1985,
Pubmed
Vale,
Intracellular transport using microtubule-based motors.
1987,
Pubmed
Yang,
Isolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.
1988,
Pubmed
