Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Cell Biol
1985 May 01;55:1143-50. doi: 10.1128/mcb.5.5.1143-1150.1985.
Show Gene links
Show Anatomy links
Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.
Morris GF
,
Marzluff WF
.
???displayArticle.abstract???
Nuclei from sea urchin blastula embryos synthesize a variety of small RNAs, one of which has identical mobility with sea urchin U1 RNA. This RNA is synthesized by RNA polymerase II and, in a hybridization-selection experiment, was selected by the cloned sea urchin U1 gene. The U1 RNA was initiated with ATP, but not GTP, in isolated nuclei with beta-S- and gamma-S-ribonucleotide triphosphates as substrates. The U1 RNA containing thiophosphate at the 5'' end was not capped but accumulated as an uncapped transcript from which the thiophosphate could be removed with calf intestinal phosphatase.
Birchmeier,
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
1983, Pubmed,
Echinobase
Birchmeier,
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
1983,
Pubmed
,
Echinobase
Blin,
Organization of the ribosomal RNA gene cluster in Lytechinus variegatus. Restriction analysis and cloning of restriction fragments.
1979,
Pubmed
,
Echinobase
Branlant,
Nucleotide sequences of nuclear U1A RNAs from chicken, rat and man.
1980,
Pubmed
Brown,
Structure of the sea urchin U1 RNA repeat.
1985,
Pubmed
,
Echinobase
Bunick,
Mechanism of RNA polymerase II--specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcripts.
1982,
Pubmed
Busch,
SnRNAs, SnRNPs, and RNA processing.
1982,
Pubmed
Card,
Sea urchin small nuclear RNA genes are organized in distinct tandemly repeating units.
1982,
Pubmed
,
Echinobase
Cohn,
Histone genes of the sea urchin (S. purpuratus) cloned in E coli: order, polarity, and strandedness of the five histone-coding and spacer regions.
1976,
Pubmed
,
Echinobase
Eliceiri,
Sensitivity of low molecular weight RNA synthesis to UV radiation.
1979,
Pubmed
Eliceiri,
Small RNAs in the nucleus and cytoplasm of HeLa cells.
1976,
Pubmed
Eliceiri,
Sensitivity to UV radiation of small nuclear RNA synthesis in mammalian cells.
1983,
Pubmed
Gurney,
Intracellular distribution of low molecular weight RNA species in HeLa cells.
1980,
Pubmed
Hagenbüchle,
Mouse beta-globin and adenovirus-2 major late transcripts are initiated at the cap site in vitro.
1981,
Pubmed
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Hofer,
The primary transcription unit of the mouse beta-major globin gene.
1981,
Pubmed
Krämer,
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
1984,
Pubmed
Lerner,
Are snRNPs involved in splicing?
1980,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Lu,
Cloning and organization of genes for 5S ribosomal RNA in the sea urchin. Lytechinus variegatus.
1981,
Pubmed
,
Echinobase
Madore,
Intracellular site of U1 small nuclear RNA processing and ribonucleoprotein assembly.
1984,
Pubmed
Manser,
Human U1 loci: genes for human U1 RNA have dramatically similar genomic environments.
1982,
Pubmed
Marzluff,
Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
1983,
Pubmed
Marzluff,
Transcription of the genes for 5S ribosomal RNA and transfer RNA in isolated mouse myeloma cell nuclei.
1974,
Pubmed
Moore,
Site-specific polyadenylation in a cell-free reaction.
1984,
Pubmed
Morris,
A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III.
1983,
Pubmed
,
Echinobase
Mount,
Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
1981,
Pubmed
Mount,
The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
1983,
Pubmed
Murphy,
Transcription of a gene for human U1 small nuclear RNA.
1982,
Pubmed
Nijhawan,
Metabolism of low molecular weight ribonucleic acids in early sea urchin embryos.
1979,
Pubmed
,
Echinobase
Padgett,
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
1983,
Pubmed
Price,
The 3' end of drosophila histone H3 mRNA is produced by a processing activity in vitro.
1984,
Pubmed
Reeve,
Guanosine 5'-O-(3-thiotriphosphate) inhibits capping of reovirus mRNA.
1982,
Pubmed
Robertson,
Identification of the RNA region transferred from a representative primer, beta-globin mRNA, to influenza mRNA during in vitro transcription.
1980,
Pubmed
Skuzeski,
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
1984,
Pubmed
Smith,
Analysis of RNA initiated in isolated mouse myeloma nuclei using purine nucleoside 5'[gamma-S]triphosphates as affinity probes.
1978,
Pubmed
Smith,
Transcription of bacteriophage lambda DNA in vitro using purine nucleoside 5'-[gamma-S]triphosphates as affinity probes for RNA chain initiation.
1978,
Pubmed
Stallcup,
Region-specific initiation of mouse mammary tumor virus RNA synthesis by endogenous RNA polymerase II in preparations of cell nuclei.
1983,
Pubmed
Washington,
A comparison of nucleoside (beta-S)triphosphates and nucleoside (gamma-S)triphosphates as suitable substrates for measuring transcription initiation in preparations of cell nuclei.
1982,
Pubmed
Watanabe-Nagasu,
Structural analysis of gene loci for rat U1 small nuclear RNA.
1983,
Pubmed
