Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Baitinger C
,
Alderton J
,
Poenie M
,
Schulman H
,
Steinhardt RA
.
???displayArticle.abstract???
The role of multifunctional Ca2+/calmodulin-dependent protein kinase (CaM kinase) in nuclear envelope breakdown (NEB) was investigated in sea urchin eggs. The eggs contain a 56-kD polypeptide which appears to be a homologue of neuronal CaM kinase. For example, it undergoes Ca2+/calmodulin-dependent autophosphorylation that converts it to a Ca2(+)-independent species, a hallmark of multifunctional CaM kinase. It is homologous to the alpha subunit of rat brain CaM kinase. Autophosphorylation and substrate phosphorylation by the sea urchin egg kinase are inhibited in vitro by CaMK(273-302), a synthetic peptide corresponding to the autoinhibitory domain of the neuronal CaM kinase. This peptide inhibited NEB when microinjected into sea urchin eggs. Only one mAb to the neuronal enzyme immunoprecipitated the 56-kD polypeptide. Only this antibody blocked or significantly delayed NEB when microinjected into sea urchin eggs. These results suggest that sea urchin eggs contain multifunctional CaM kinase, and that this enzyme is involved in the control of NEB during mitotic division.
Bennett,
Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning.
1987, Pubmed
Bennett,
Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning.
1987,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bulleit,
Conserved and variable regions in the subunits of brain type II Ca2+/calmodulin-dependent protein kinase.
1988,
Pubmed
Chou,
Purification and characterization of a sea urchin egg Ca2+-calmodulin-dependent kinase with myosin light chain phosphorylating activity.
1986,
Pubmed
,
Echinobase
Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977,
Pubmed
Dinsmore,
Calcium and calmodulin-dependent phosphorylation of a 62 kd protein induces microtubule depolymerization in sea urchin mitotic apparatuses.
1988,
Pubmed
,
Echinobase
Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed
,
Echinobase
Ey,
Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.
1978,
Pubmed
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Hanley,
Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II.
1987,
Pubmed
Hanson,
Expression of a multifunctional Ca2+/calmodulin-dependent protein kinase and mutational analysis of its autoregulation.
1989,
Pubmed
Hepler,
Calcium transients during mitosis: observations in flux.
1989,
Pubmed
Hunt,
Maturation promoting factor, cyclin and the control of M-phase.
1989,
Pubmed
Kelly,
Active site-directed inhibition of Ca2+/calmodulin-dependent protein kinase type II by a bifunctional calmodulin-binding peptide.
1988,
Pubmed
Kennel,
Binding of monoclonal antibody to protein antigen in fluid phase or bound to solid supports.
1982,
Pubmed
Lin,
Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.
1987,
Pubmed
Lou,
Distinct autophosphorylation sites sequentially produce autonomy and inhibition of the multifunctional Ca2+/calmodulin-dependent protein kinase.
1989,
Pubmed
Malinow,
Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTP.
1989,
Pubmed
Miller,
Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: a Ca2+-triggered molecular switch.
1986,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
Payne,
Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains.
1988,
Pubmed
Poenie,
Changes of free calcium levels with stages of the cell division cycle.
1985,
Pubmed
,
Echinobase
Poenie,
Calcium rises abruptly and briefly throughout the cell at the onset of anaphase.
1986,
Pubmed
Roskoski,
Assays of protein kinase.
1983,
Pubmed
Scholz,
Developmental changes in Ca2+/calmodulin-dependent protein kinase II in cultures of hippocampal pyramidal neurons and astrocytes.
1988,
Pubmed
Schulman,
The multifunctional Ca2+/calmodulin-dependent protein kinase.
1988,
Pubmed
Schulman,
Multifunctional Ca2+/calmodulin-dependent protein kinase: domain structure and regulation.
1989,
Pubmed
Schulman,
Ca2+-dependent protein phosphorylation system in membranes from various tissues, and its activation by "calcium-dependent regulator".
1978,
Pubmed
Schulman,
Phosphorylation of microtubule-associated proteins by a Ca2+/calmodulin-dependent protein kinase.
1984,
Pubmed
Silver,
Nuclear envelope breakdown and mitosis in sand dollar embryos is inhibited by microinjection of calcium buffers in a calcium-reversible fashion, and by antagonists of intracellular Ca2+ channels.
1989,
Pubmed
,
Echinobase
Steinhardt,
Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Tobimatsu,
Molecular cloning of the cDNA encoding the third polypeptide (gamma) of brain calmodulin-dependent protein kinase II.
1988,
Pubmed
Tobimatsu,
Tissue-specific expression of four types of rat calmodulin-dependent protein kinase II mRNAs.
1989,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Twigg,
Translational control of InsP3-induced chromatin condensation during the early cell cycles of sea urchin embryos.
1988,
Pubmed
,
Echinobase
Whitaker,
Calcium and cell cycle control.
1990,
Pubmed
,
Echinobase
