Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Early expression of a collagenase-like hatching enzyme gene in the sea urchin embryo.
Lepage T
,
Gache C
.
???displayArticle.abstract???
The hatching enzyme is a developmentally regulated protease secreted at the blastula stage by the sea urchin embryo to digest its protective envelope. A nearly full-length cDNA clone (HE6) encoding the entire sequence of the hatching enzyme was isolated from a prehatching blastula lambda gt11 cDNA library. The 1761 bp open reading frame codes for a preprohatching enzyme with an 18 amino acid signal sequence, a 148 amino acid activation peptide and a 421 amino acid mature enzyme which has homologies with the mammalian collagenases. Transcripts of the hatching enzyme gene are not detected in the unfertilized egg, they accumulate during the cleavage stages and disappear at hatching. This transient expression results from a transcriptional control. Thus the hatching enzyme mRNA is not a maternal gene product but a transcript synthesized at a very early stage from the zygotic genome.
Aebersold,
Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis.
1986, Pubmed
Aebersold,
Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis.
1986,
Pubmed
Alexander,
Proteinases and extracellular matrix remodeling.
1989,
Pubmed
Angel,
Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
1987,
Pubmed
Angerer,
Expression of a collagen gene in mesenchyme lineages of the Strongylocentrotus purpuratus embryo.
1988,
Pubmed
,
Echinobase
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Barrett,
Maternal characteristics of hatching enzymes in hybrid sea urchin embryos.
1969,
Pubmed
,
Echinobase
Barrett,
Hatching enzyme of the sea urchin Strongylocentrotus purpuratus.
1976,
Pubmed
,
Echinobase
Benson,
A lineage-specific gene encoding a major matrix protein of the sea urchin embryo spicule. I. Authentication of the cloned gene and its developmental expression.
1987,
Pubmed
,
Echinobase
Brenner,
Genes for extracellular-matrix-degrading metalloproteinases and their inhibitor, TIMP, are expressed during early mammalian development.
1989,
Pubmed
Britten,
The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus.
1978,
Pubmed
,
Echinobase
Bruskin,
Accumulation in embryogenesis of five mRNAs enriched in the ectoderm of the sea urchin pluteus.
1981,
Pubmed
,
Echinobase
Cavener,
Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates.
1987,
Pubmed
Chiu,
The c-Fos protein interacts with c-Jun/AP-1 to stimulate transcription of AP-1 responsive genes.
1988,
Pubmed
Chou,
Empirical predictions of protein conformation.
1978,
Pubmed
Collier,
H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen.
1988,
Pubmed
Costanzi,
Fast blots: immobilization of DNA and RNA from cells.
1987,
Pubmed
Devault,
Amino acid sequence of rabbit kidney neutral endopeptidase 24.11 (enkephalinase) deduced from a complementary DNA.
1987,
Pubmed
Dolecki,
Stage-specific expression of a homeo box-containing gene in the non-segmented sea urchin embryo.
1986,
Pubmed
,
Echinobase
Edelman,
Protein serine/threonine kinases.
1987,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
GROSS,
Effects of actinomycin D on macromolecule synthesis and early development in sea urchin eggs.
1963,
Pubmed
,
Echinobase
Garfinkel,
DNA sequences, gene regulation and modular protein evolution in the Drosophila 68C glue gene cluster.
1983,
Pubmed
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Goldberg,
Human fibroblast collagenase. Complete primary structure and homology to an oncogene transformation-induced rat protein.
1986,
Pubmed
Grant,
Developmental expression of cell-surface (glyco)proteins involved in gastrulation and spicule formation in sea urchin embryos.
1985,
Pubmed
,
Echinobase
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Harkey,
Coordinate accumulation of five transcripts in the primary mesenchyme during skeletogenesis in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Hattori,
Structure and chromosomal localization of the functional intronless human JUN protooncogene.
1988,
Pubmed
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Jongeneel,
A unique signature identifies a family of zinc-dependent metallopeptidases.
1989,
Pubmed
Kester,
Comparison of the structures of carboxypeptidase A and thermolysin.
1977,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Lawler,
The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins.
1986,
Pubmed
Lee,
Activation of sea urchin actin genes during embryogenesis. Measurement of transcript accumulation from five different genes in Strongylocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Lepage,
Purification and characterization of the sea urchin embryo hatching enzyme.
1989,
Pubmed
,
Echinobase
Lipman,
Rapid and sensitive protein similarity searches.
1985,
Pubmed
Marck,
'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.
1988,
Pubmed
Matrisian,
Isolation of the oncogene and epidermal growth factor-induced transin gene: complex control in rat fibroblasts.
1986,
Pubmed
Mierendorf,
Gene isolation by screening lambda gt11 libraries with antibodies.
1987,
Pubmed
Mierendorf,
Direct sequencing of denatured plasmid DNA.
1987,
Pubmed
Morris,
A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III.
1983,
Pubmed
,
Echinobase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
Nevins,
Isolation and analysis of nuclear RNA.
1987,
Pubmed
Nocente-McGrath,
Endo16, a lineage-specific protein of the sea urchin embryo, is first expressed just prior to gastrulation.
1989,
Pubmed
,
Echinobase
Olmsted,
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
1981,
Pubmed
Pearson,
Improved tools for biological sequence comparison.
1988,
Pubmed
Ringwald,
The structure of cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion.
1987,
Pubmed
Ruoslahti,
Fibronectin and its receptors.
1988,
Pubmed
Sanchez-Lopez,
Structure-function relationships in the collagenase family member transin.
1988,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schönthal,
Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters.
1988,
Pubmed
Shaw,
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
1986,
Pubmed
Showman,
Removal of the fertilization membrane of sea urchin embryos employing aminotriazole.
1979,
Pubmed
,
Echinobase
Soeiro,
Competition hybridization by "pre-saturation" of HeLa cell DNA.
1969,
Pubmed
Spinelli,
Evidences of two different sets of histone genes active during embryogenesis of the sea urchin Paracentrotus lividus.
1979,
Pubmed
,
Echinobase
Stricklin,
Human skin fibroblast procollagenase: mechanisms of activation by organomercurials and trypsin.
1983,
Pubmed
Stuart,
Alpha-lactalbumin possesses a novel calcium binding loop.
1986,
Pubmed
Tabor,
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
1987,
Pubmed
Takahashi,
Structure of human hemopexin: O-glycosyl and N-glycosyl sites and unusual clustering of tryptophan residues.
1984,
Pubmed
Takeuchi,
Purification and characterization of hatching enzyme of Strongylocentrotus intermedius.
1979,
Pubmed
,
Echinobase
Terman,
Translation-level control of protein synthesis during early development.
1965,
Pubmed
Uzman,
The role of RNA polymerase initiation and elongation in control of total RNA and histone mRNA synthesis in sea urchin embryos.
1984,
Pubmed
,
Echinobase
Wessel,
Gastrulation in the sea urchin embryo requires the deposition of crosslinked collagen within the extracellular matrix.
1987,
Pubmed
,
Echinobase
Whiteley,
Changing populations of reiterated DNA transcripts during early echinoderm development.
1975,
Pubmed
,
Echinobase
Whitham,
Comparison of human stromelysin and collagenase by cloning and sequence analysis.
1986,
Pubmed
Yang,
Structure and tissue-specific developmental expression of a sea urchin arylsulfatase gene.
1989,
Pubmed
,
Echinobase
Yang,
Unusual pattern of accumulation of mRNA encoding EGF-related protein in sea urchin embryos.
1989,
Pubmed
,
Echinobase
Young,
Yeast RNA polymerase II genes: isolation with antibody probes.
1983,
Pubmed
von Heijne,
Signal sequences. The limits of variation.
1985,
Pubmed
von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed
