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Echinobase
ECB-ART-31315
Dev Biol 1991 Dec 01;1482:625-30. doi: 10.1016/0012-1606(91)90280-g.
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Sea urchin USF: a helix-loop-helix protein active in embryonic ectoderm cells.

Kozlowski MT , Gan L , Venuti JM , Sawadogo M , Klein WH .


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We previously characterized a DNA-binding factor in nuclear extracts of Strongylocentrotus purpuratus embryos that bound Spec gene promoters, was ectoderm specific, and had properties similar to the vertebrate transcription factor USF. Here we describe a cDNA clone, suUSF, isolated from an S. purpuratus cDNA library, with sequence homology to human USF. Spec gene promoter fragments formed sequence-specific complexes with suUSF, and antibodies against suUSF inhibited binding activity in nuclear extracts. Reaction of USF-site containing probes with filter-bound nuclear proteins demonstrated that suUSF binding activity was enriched in ectoderm cells, and immunoblotting showed a similar ectoderm enrichment. These data demonstrated that suUSF was responsible for the ectoderm-specific activity observed in sea urchin extracts.

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Genes referenced: LOC100887844 usf2