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Nucleic Acids Res
1992 Jul 25;2014:3743-51. doi: 10.1093/nar/20.14.3743.
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Two promoter elements are necessary and sufficient for expression of the sea urchin U1 snRNA gene.
Wendelburg BJ
,
Marzluff WF
.
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The essential elements of the sea urchin L. variegatus U1 snRNA promoter were mapped by microinjection of a U1 maxigene into sea urchin zygotes. Two elements are required for expression: a distal sequence element (DSE) located between -318 and -300 and a proximal sequence element (PSE) centered at -55. Removal or alteration of other sequences conserved in different sea urchin snRNA U1 genes, including deletion of all sequence between -90 and -273, did not affect the expression. Sequences around the start site were not required for expression. Deletion of nucleotides between the PSE and the start site resulted in initiation inside the U1 coding region, suggesting that the PSE determines the start site of transcription. There is no obvious similarity between the sequences required for the sea urchin U1 snRNA expression and the sequences required for the expression of other sea urchin snRNAs.
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985, Pubmed
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985,
Pubmed
Ares,
Distinct factors with Sp1 and NF-A specificities bind to adjacent functional elements of the human U2 snRNA gene enhancer.
1987,
Pubmed
Carbon,
A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
1987,
Pubmed
Ciliberto,
Properties of a U1 RNA enhancer-like sequence.
1987,
Pubmed
Colin,
Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes.
1988,
Pubmed
,
Echinobase
De Lorenzi,
Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
1986,
Pubmed
,
Echinobase
Gruber,
Isolation of an active gene and of two pseudogenes for mouse U7 small nuclear RNA.
1991,
Pubmed
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Howard,
Functional, developmentally expressed genes for mouse U1a and U1b snRNAs contain both conserved and non-conserved transcription signals.
1986,
Pubmed
Korf,
Developmental and tissue-specific expression of U4 small nuclear RNA genes.
1988,
Pubmed
Kunkel,
Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used.
1988,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Lund,
Differential control of U1 small nuclear RNA expression during mouse development.
1985,
Pubmed
Mangin,
Human U2 small nuclear RNA genes contain an upstream enhancer.
1986,
Pubmed
McMahon,
Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis.
1985,
Pubmed
,
Echinobase
Nash,
A developmental switch in sea urchin U1 RNA.
1989,
Pubmed
,
Echinobase
Parry,
The Xenopus U2 gene PSE is a single, compact, element required for transcription initiation and 3' end formation.
1989,
Pubmed
Phillips,
Nucleotide sequence of the mouse U7 snRNA gene.
1991,
Pubmed
Roebuck,
Octamer and SPH motifs in the U1 enhancer cooperate to activate U1 RNA gene expression.
1990,
Pubmed
Santiago,
Expression of the U1 RNA gene repeat during early sea urchin development: evidence for a switch in U1 RNA genes during development.
1989,
Pubmed
,
Echinobase
Southgate,
In vivo and in vitro expression of U7 snRNA genes: cis- and trans-acting elements required for RNA polymerase II-directed transcription.
1989,
Pubmed
,
Echinobase
Stefanovic,
Characterization of two developmentally regulated sea urchin U2 small nuclear RNA promoters: a common required TATA sequence and independent proximal and distal elements.
1992,
Pubmed
,
Echinobase
Stevenson,
A conserved region in the sea urchin U1 snRNA promoter interacts with a developmentally regulated factor.
1992,
Pubmed
,
Echinobase
Strub,
Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
1986,
Pubmed
,
Echinobase
Suter-Crazzolara,
Organization and transient expression of the gene for human U11 snRNA.
1991,
Pubmed
Tanaka,
Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements.
1988,
Pubmed
Thomas,
The spliceosomal snRNAs of Caenorhabditis elegans.
1990,
Pubmed
Vankan,
Structure and expression of the U5 snRNA gene of Arabidopsis thaliana. Conserved upstream sequence elements in plant U-RNA genes.
1988,
Pubmed
Yu,
Structure and expression of a second sea urchin U1 RNA gene repeat.
1986,
Pubmed
,
Echinobase
Yu,
The U1 snRNA gene repeat from the sea urchin (Strongylocentrotus purpuratus): the 70 kilobase tandem repeat ends directly 3' to a U1 gene.
1991,
Pubmed
,
Echinobase
