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Members of the USF family of helix-loop-helix proteins bind DNA as homo- as well as heterodimers.
Sirito M
,
Walker S
,
Lin Q
,
Kozlowski MT
,
Klein WH
,
Sawadogo M
.
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We have isolated human cDNA clones for USF2, a new member of the upstream stimulatory factor (USF) family of transcription factors. Analysis of these clones revealed the existence of highly conserved elements in the C terminal region of all USF proteins. These include the basic region, helix-loop-helix (HLH) motif, and, in the case of the human proteins, the C-terminal leucine repeat (LR). In addition, a highly conserved USF-specific domain is located immediately upstream of the basic region. Using in vitro translated proteins, we found that all members of the USF family bound DNA as dimers. The N-terminal portion of USF, including the USF-specific domain, was entirely dispensable for dimer formation and DNA-binding. However, deletion mutants of USF2 lacking the LR were deficient in DNA-binding activity. Interestingly, each of the USF proteins could form functional heterodimers with the other family members, including the sea urchin USF, which does not have a LR motif. This indicates that the conserved LR in human USF is not required for dimer formation, and influences only indirectly DNA-binding.
Beckmann,
The leucine zipper of TFE3 dictates helix-loop-helix dimerization specificity.
1991, Pubmed
Beckmann,
The leucine zipper of TFE3 dictates helix-loop-helix dimerization specificity.
1991,
Pubmed
Beckmann,
TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif.
1990,
Pubmed
Blackwell,
Sequence-specific DNA binding by the c-Myc protein.
1990,
Pubmed
Blackwood,
Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.
1991,
Pubmed
Carr,
A helix-loop-helix protein related to the immunoglobulin E box-binding proteins.
1990,
Pubmed
Carthew,
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
1985,
Pubmed
Carthew,
The major late transcription factor binds to and activates the mouse metallothionein I promoter.
1987,
Pubmed
Chang,
Adeno-associated virus P5 promoter contains an adenovirus E1A-inducible element and a binding site for the major late transcription factor.
1989,
Pubmed
Chodosh,
The adenovirus major late transcription factor activates the rat gamma-fibrinogen promoter.
1987,
Pubmed
Dang,
Involvement of the 'leucine zipper' region in the oligomerization and transforming activity of human c-myc protein.
1989,
Pubmed
Fisher,
TFEB has DNA-binding and oligomerization properties of a unique helix-loop-helix/leucine-zipper family.
1991,
Pubmed
Greaves,
Sequence, function, and regulation of the Vmw65 gene of herpes simplex virus type 2.
1991,
Pubmed
Gregor,
The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer.
1990,
Pubmed
Hu,
Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity.
1990,
Pubmed
Kaulen,
The Xenopus B1 factor is closely related to the mammalian activator USF and is implicated in the developmental regulation of TFIIIA gene expression.
1991,
Pubmed
Kerkhoff,
Sequence-specific DNA binding by Myc proteins.
1991,
Pubmed
Kozlowski,
Sea urchin USF: a helix-loop-helix protein active in embryonic ectoderm cells.
1991,
Pubmed
,
Echinobase
Lemaigre,
Evidence that the upstream stimulatory factor and the Sp1 transcription factor bind in vitro to the promoter of the human-growth-hormone gene.
1989,
Pubmed
Miyamoto,
Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter.
1985,
Pubmed
Murre,
A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
1989,
Pubmed
Potter,
The upstream stimulatory factor binds to and activates the promoter of the rat class I alcohol dehydrogenase gene.
1991,
Pubmed
Prendergast,
Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation.
1991,
Pubmed
Prendergast,
Methylation-sensitive sequence-specific DNA binding by the c-Myc basic region.
1991,
Pubmed
Reach,
Transcription from the adenovirus major late promoter uses redundant activating elements.
1991,
Pubmed
Roy,
Cooperative interaction of an initiator-binding transcription initiation factor and the helix-loop-helix activator USF.
1991,
Pubmed
Rüther,
Easy identification of cDNA clones.
1983,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sato,
Interaction of upstream stimulatory factor with the human heme oxygenase gene promoter.
1990,
Pubmed
Sawadogo,
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
1985,
Pubmed
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. II. DNA binding properties and transcriptional activity of the purified HeLa USF.
1988,
Pubmed
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.
1988,
Pubmed
Scheidereit,
A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.
1988,
Pubmed
Scotto,
Positive and negative regulation of the gene for transcription factor IIIA in Xenopus laevis oocytes.
1989,
Pubmed
Van Dyke,
Single-step purification of bacterially expressed polypeptides containing an oligo-histidine domain.
1992,
Pubmed
Vinson,
Scissors-grip model for DNA recognition by a family of leucine zipper proteins.
1989,
Pubmed
Voronova,
Mutations that disrupt DNA binding and dimer formation in the E47 helix-loop-helix protein map to distinct domains.
1990,
Pubmed
Zimmerman,
Expression and function of myc family genes.
1990,
Pubmed
