Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1979 Oct 01;831:109-15. doi: 10.1083/jcb.83.1.109.
Show Gene links
Show Anatomy links
Isolation and characterization of two forms of a cytoskeleton.
Edds KT
.
???displayArticle.abstract???
Isolated petaloid coelomocytes from the sea urchin Strongylocentrotus droebachiensis transform to a filopodial morphology in hypotonic media. Electron micrographs of negatively stained Triton-insoluble cytoskeletons show that the petaloid form consists of a loose net of microfilaments while the filopodial form consists of paracrystalline bundles of microfilaments. Actin is the major protein of both forms of the cytoskeleton. Additional polypeptides have molecular weights of approximately 220,000, 64,000, 57,000, and 27,000 daltons. Relative to actin the filopodial cytoskeletons have an average of 2.5 times as much 57k polypeptide as the petaloid cytoskeletons. Treatment with 0.25 M NaCl dissociates the filament bundles into individual actin filaments free of the actin-associated polypeptides. Thus, one or more of these actin-associated polypeptides may be responsible for crosslinking the actin filaments into bundles and maintaining the three-dimensional nature of the cytoskeletons.
Begg,
The visualization of actin filament polarity in thin sections. Evidence for the uniform polarity of membrane-associated filaments.
1978, Pubmed,
Echinobase
Begg,
The visualization of actin filament polarity in thin sections. Evidence for the uniform polarity of membrane-associated filaments.
1978,
Pubmed
,
Echinobase
Brown,
Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction.
1976,
Pubmed
Bryan,
Separation and interaction of the major components of sea urchin actin gel.
1978,
Pubmed
,
Echinobase
Buckley,
Cytoplasmic fibrils in living cultured cells. A light and electron microscope study.
1967,
Pubmed
Burgess,
Polarized bundles of actin filaments within microvilli of fertilized sea urchin eggs.
1977,
Pubmed
,
Echinobase
DeRosier,
Structure of actin-containing filaments from two types of non-muscle cells.
1977,
Pubmed
,
Echinobase
Edds,
Dynamic aspects of filopodial formation by reorganization of microfilaments.
1977,
Pubmed
,
Echinobase
Fairbanks,
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
1971,
Pubmed
Herman,
Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells.
1979,
Pubmed
Ishikawa,
Formation of arrowhead complexes with heavy meromyosin in a variety of cell types.
1969,
Pubmed
Kane,
Actin polymerization and interaction with other proteins in temperature-induced gelation of sea urchin egg extracts.
1976,
Pubmed
,
Echinobase
Lazarides,
Actin antibody: the specific visualization of actin filaments in non-muscle cells.
1974,
Pubmed
Mooseker,
Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells.
1975,
Pubmed
Osborn,
The detertent-resistant cytoskeleton of tissue culture cells includes the nucleus and the microfilament bundles.
1977,
Pubmed
Stossel,
Interactions between actin, myosin, and an actin-binding protein from rabbit alveolar macrophages. Alveolar macrophage myosin Mg-2+-adenosine triphosphatase requires a cofactor for activation by actin.
1975,
Pubmed
Tilney,
Actin filaments in the acrosomal reaction of Limulus sperm. Motion generated by alterations in the packing of the filaments.
1975,
Pubmed
Trotter,
Intracellular fibres in cultured cells: analysis by scanning and transmission electron microscopy and by SDS-polyacrylamide gel electrophoresis.
1978,
Pubmed
Weber,
Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.
1972,
Pubmed
