Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
The Nodal signaling pathway is known from earlier work to be an essential mediator of oral ectoderm specification in the sea urchin embryo, and indirectly, of aboral ectoderm specification as well. Following expression of the Nodal ligand in the future oral ectoderm during cleavage, a sequence of regulatory gene activations occur within this territory which depend directly or indirectly on nodal gene expression. Here we describe additional regulatory genes that contribute to the oral ectoderm regulatory state during specification in Strongylocentrotus purpuratus, and show how their spatial expression changes dynamically during development. By means of system wide perturbation analyses we have significantly improved current knowledge of the epistatic relations among the regulatory genes of the oral ectoderm. From these studies there emerge diverse circuitries relating downstream regulatory genes directly and indirectly to Nodal signaling. A key intermediary regulator, the role of which had not previously been discerned, is the not gene. In addition to activating several genes earlier described as targets of Nodal signaling, the not gene product acts to repress other oral ectoderm genes, contributing crucially to the bilateral spatial organization of the embryonic oral ectoderm.
Bergeron,
Oral-aboral patterning and gastrulation of sea urchin embryos depend on sulfated glycosaminoglycans.
2011, Pubmed,
Echinobase
Bergeron,
Oral-aboral patterning and gastrulation of sea urchin embryos depend on sulfated glycosaminoglycans.
2011,
Pubmed
,
Echinobase Bolouri,
Transcriptional regulatory cascades in development: initial rates, not steady state, determine network kinetics.
2003,
Pubmed
,
Echinobase Bolouri,
The gene regulatory network basis of the "community effect," and analysis of a sea urchin embryo example.
2010,
Pubmed
,
Echinobase Cameron,
SpBase: the sea urchin genome database and web site.
2009,
Pubmed
,
Echinobase Cameron,
Macromere cell fates during sea urchin development.
1991,
Pubmed
,
Echinobase Cameron,
Segregation of oral from aboral ectoderm precursors is completed at fifth cleavage in the embryogenesis of Strongylocentrotus purpuratus.
1990,
Pubmed
,
Echinobase Chen,
The dynamic gene expression patterns of transcription factors constituting the sea urchin aboral ectoderm gene regulatory network.
2011,
Pubmed
,
Echinobase Coffman,
Oral-aboral axis specification in the sea urchin embryo. I. Axis entrainment by respiratory asymmetry.
2001,
Pubmed
,
Echinobase Coffman,
Oral-aboral axis specification in the sea urchin embryo II. Mitochondrial distribution and redox state contribute to establishing polarity in Strongylocentrotus purpuratus.
2004,
Pubmed
,
Echinobase Coluccio,
Oxygen, pH, and oral-aboral axis specification in the sea urchin embryo.
2011,
Pubmed
,
Echinobase Croce,
Expression pattern of Brachyury in the embryo of the sea urchin Paracentrotus lividus.
2001,
Pubmed
,
Echinobase Duboc,
Nodal and BMP2/4 signaling organizes the oral-aboral axis of the sea urchin embryo.
2004,
Pubmed
,
Echinobase Duboc,
Nodal and BMP2/4 pattern the mesoderm and endoderm during development of the sea urchin embryo.
2010,
Pubmed
,
Echinobase Duboc,
Lefty acts as an essential modulator of Nodal activity during sea urchin oral-aboral axis formation.
2008,
Pubmed
,
Echinobase Duloquin,
Localized VEGF signaling from ectoderm to mesenchyme cells controls morphogenesis of the sea urchin embryo skeleton.
2007,
Pubmed
,
Echinobase Flowers,
Nodal/activin signaling establishes oral-aboral polarity in the early sea urchin embryo.
2004,
Pubmed
,
Echinobase Lapraz,
Patterning of the dorsal-ventral axis in echinoderms: insights into the evolution of the BMP-chordin signaling network.
2009,
Pubmed
,
Echinobase Longabaugh,
Visualization, documentation, analysis, and communication of large-scale gene regulatory networks.
2009,
Pubmed Materna,
High accuracy, high-resolution prevalence measurement for the majority of locally expressed regulatory genes in early sea urchin development.
2010,
Pubmed
,
Echinobase Minokawa,
Expression patterns of four different regulatory genes that function during sea urchin development.
2004,
Pubmed
,
Echinobase Nam,
Cis-regulatory control of the nodal gene, initiator of the sea urchin oral ectoderm gene network.
2007,
Pubmed
,
Echinobase Oliveri,
Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo.
2006,
Pubmed
,
Echinobase Otim,
SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis.
2004,
Pubmed
,
Echinobase Peter,
A gene regulatory network controlling the embryonic specification of endoderm.
2011,
Pubmed
,
Echinobase Peterson,
Expression pattern of Brachyury and Not in the sea urchin: comparative implications for the origins of mesoderm in the basal deuterostomes.
1999,
Pubmed
,
Echinobase Range,
Cis-regulatory analysis of nodal and maternal control of dorsal-ventral axis formation by Univin, a TGF-beta related to Vg1.
2007,
Pubmed
,
Echinobase Ransick,
Detection of mRNA by in situ hybridization and RT-PCR.
2004,
Pubmed
,
Echinobase Ransick,
Late specification of Veg1 lineages to endodermal fate in the sea urchin embryo.
1998,
Pubmed
,
Echinobase Saudemont,
Ancestral regulatory circuits governing ectoderm patterning downstream of Nodal and BMP2/4 revealed by gene regulatory network analysis in an echinoderm.
2010,
Pubmed
,
Echinobase Su,
A perturbation model of the gene regulatory network for oral and aboral ectoderm specification in the sea urchin embryo.
2009,
Pubmed
,
Echinobase Tu,
Sea urchin Forkhead gene family: phylogeny and embryonic expression.
2006,
Pubmed
,
Echinobase Yaguchi,
Sp-Smad2/3 mediates patterning of neurogenic ectoderm by nodal in the sea urchin embryo.
2007,
Pubmed
,
Echinobase
