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J Cell Biol
1996 Jan 01;1321-2:125-35. doi: 10.1083/jcb.132.1.125.
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Initial triggering of M-phase in starfish oocytes: a possible novel component of maturation-promoting factor besides cdc2 kinase.
Okumura E, Sekiai T, Hisanaga S, Tachibana K, Kishimoto T.
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G2-phase-arrested immature starfish oocytes contain inactive cdc2 kinase and cdc25 phosphatase, and an inactivator for cdc2 kinase. In this system, we have studied how the regulatory balance is apped toward the initial activation of cdc2 kinase. During the hormone-dependent period (Guerrier, P., and M. Doree, 1975. Dev. Biol. 47:341-348), p34cdc2 and cdc25 protein are already converted, though not fully, to active forms, whereas the inactivators for cdc2 kinase and cdc25 phosphatase are able to exhibit their activities if the hormone were removed. We produced "triggered oocytes," in which due to a neutralizing anticdc25 antibody, the activation of cdc2 kinase is prevented out cdc25 protein is phosphorylated slightly after the maturation-inducing hormonal stimulus. In contrast to control immature oocytes, in triggered oocytes the injected cdc2 kinase is not inactivated, and accordingly the level of cdc2 kinase activity required for meiosis reinitiation is much less. These results imply the presence of a cdc2 kinase activity-independent process(es) that suppresses the inactivator for cdc2 kinase and initially phosphorylates cdc25 protein, although this process is reversible during the initial activation of cdc2 kinase. At the most initial triggering of M-phase, the cdc2 kinase activity-independent process might trip the switch leading to the initial activation of cdc2 kinase. Thereafter, in parallel, the cdc2 kinase-dependent feedback loops described by others may cause further increase in cdc2 kinase activity. We propose that a putative suppressor, which downregulates the inactivator for cdc2 kinase independently of nuclear components, might be a previously unrecognized component of maturation-promoting factor.
Atherton-Fessler,
Cell cycle regulation of the p34cdc2 inhibitory kinases.
1994, Pubmed
Atherton-Fessler,
Cell cycle regulation of the p34cdc2 inhibitory kinases.
1994,
Pubmed Clarke,
Dephosphorylation of cdc25-C by a type-2A protein phosphatase: specific regulation during the cell cycle in Xenopus egg extracts.
1993,
Pubmed Coleman,
Negative regulation of the wee1 protein kinase by direct action of the nim1/cdr1 mitotic inducer.
1993,
Pubmed Devault,
Cyclin A potentiates maturation-promoting factor activation in the early Xenopus embryo via inhibition of the tyrosine kinase that phosphorylates cdc2.
1992,
Pubmed
,
Echinobase Dorée,
Control of M-phase by maturation-promoting factor.
1990,
Pubmed Galaktionov,
Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins.
1991,
Pubmed Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed Guerrier,
Hormonal control of reinitiation of meiosis in starfish. The requirement of 1-methyladenine during nuclear maturation.
1975,
Pubmed
,
Echinobase Hisanaga,
Porcine brain neurofilament-H tail domain kinase: its identification as cdk5/p26 complex and comparison with cdc2/cyclin B kinase.
1995,
Pubmed Hoffmann,
Phosphorylation and activation of human cdc25-C by cdc2--cyclin B and its involvement in the self-amplification of MPF at mitosis.
1993,
Pubmed Hunt,
Maturation promoting factor, cyclin and the control of M-phase.
1989,
Pubmed Izumi,
Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
1992,
Pubmed Izumi,
Elimination of cdc2 phosphorylation sites in the cdc25 phosphatase blocks initiation of M-phase.
1993,
Pubmed Izumi,
Phosphorylation and activation of the Xenopus Cdc25 phosphatase in the absence of Cdc2 and Cdk2 kinase activity.
1995,
Pubmed Jessus,
Oscillation of MPF is accompanied by periodic association between cdc25 and cdc2-cyclin B.
1992,
Pubmed King,
Mitosis in transition.
1994,
Pubmed Kishimoto,
Extraction and preliminary characterization of maturation-promoting factor from starfish oocytes.
1986,
Pubmed
,
Echinobase Kishimoto,
Role of germinal vesicle material in producing maturation-promoting factor in starfish oocyte.
1981,
Pubmed
,
Echinobase Kishimoto,
Cell reproduction: induction of M-phase events by cyclin-dependent cdc2 kinase.
1994,
Pubmed Kishimoto,
Microinjection and cytoplasmic transfer in starfish oocytes.
1986,
Pubmed
,
Echinobase Kishimoto,
Cytoplasmic factor responsible for germinal vesicle breakdown and meiotic maturation in starfish oocyte.
1976,
Pubmed
,
Echinobase Kitagawa,
Butyrolactone I, a selective inhibitor of cdk2 and cdc2 kinase.
1993,
Pubmed Kornbluth,
Membrane localization of the kinase which phosphorylates p34cdc2 on threonine 14.
1994,
Pubmed Kuang,
cdc25 is one of the MPM-2 antigens involved in the activation of maturation-promoting factor.
1994,
Pubmed Kuang,
At least two kinases phosphorylate the MPM-2 epitope during Xenopus oocyte maturation.
1993,
Pubmed Kuang,
Multiple forms of maturation-promoting factor in unfertilized Xenopus eggs.
1991,
Pubmed Kumagai,
The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system.
1991,
Pubmed Kumagai,
Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
1992,
Pubmed Kusubata,
p13suc1 suppresses the catalytic function of p34cdc2 kinase for intermediate filament proteins, in vitro.
1992,
Pubmed Labbé,
M phase-specific cdc2 kinase: preparation from starfish oocytes and properties.
1991,
Pubmed
,
Echinobase Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
,
Echinobase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed Maller,
Mitotic control.
1991,
Pubmed Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed McGowan,
Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15.
1993,
Pubmed McGowan,
Cell cycle regulation of human WEE1.
1995,
Pubmed Millar,
The cdc25 M-phase inducer: an unconventional protein phosphatase.
1992,
Pubmed Morgan,
Principles of CDK regulation.
1995,
Pubmed Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed Ookata,
Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes.
1992,
Pubmed
,
Echinobase Parker,
Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.
1992,
Pubmed Parker,
Phosphorylation and inactivation of the mitotic inhibitor Wee1 by the nim1/cdr1 kinase.
1993,
Pubmed Picard,
The role of the germinal vesicle in producing maturation-promoting factor (MPF) as revealed by the removal and transplantation of nuclear material in starfish oocytes.
1984,
Pubmed
,
Echinobase Picard,
The cell cycle can occur in starfish oocytes and embryos without the production of transferable MPF (maturation-promoting factor).
1988,
Pubmed
,
Echinobase Picard,
Okadaic acid mimics a nuclear component required for cyclin B-cdc2 kinase microinjection to drive starfish oocytes into M phase.
1991,
Pubmed
,
Echinobase Sebastian,
Cdc25M2 activation of cyclin-dependent kinases by dephosphorylation of threonine-14 and tyrosine-15.
1993,
Pubmed Solomon,
The function(s) of CAK, the p34cdc2-activating kinase.
1994,
Pubmed Strausfeld,
Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein.
1991,
Pubmed
,
Echinobase Tang,
Two distinct mechanisms for negative regulation of the Wee1 protein kinase.
1993,
Pubmed Watanabe,
Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle.
1995,
Pubmed Westendorf,
Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope.
1994,
Pubmed Wu,
Nim1 kinase promotes mitosis by inactivating Wee1 tyrosine kinase.
1993,
Pubmed Zheng,
Functional analysis of the P box, a domain in cyclin B required for the activation of Cdc25.
1993,
Pubmed
