Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
The subcellular distribution and regulation of MAP kinase isoforms in chicken hepatoma DU249 cells was investigated with antibodies directed against peptides patterned after sequences in the mitogen-activated protein (MAP) kinases, sea star p44mpk, and rat p44erk1. MonoQ chromatography of cytosol from these cells afforded the resolution of at least four peaks of myelin basic protein (MBP) phosphotransferase activity, but only one of these (peak II) was stimulated in extracts from phorbol ester-treated cells. A 40- to 41-kDa (p41) doublet on Western blots detected with three different MAP kinase antibodies was coincident with peak II, and it probably corresponded to the avian homolog of p42mapk/erk2. Immunofluorescent studies with DU249 cells and chicken embryo fibroblasts revealed that most of the cross-reactive protein with at least two different MAP kinase antibodies was distributed in the nucleus. Subcellular fractionation studies confirmed a predominantly nuclear localization for p41 MAP kinase. Nocodazole arrest of DU249 cells was exploited for the detection of an M-phase-activated MBP kinase that was resolved from p41 MAP kinase by phenyl-Superose chromatography. Western blotting analysis with antibodies for the cdc2-encoded protein kinase and p13suc1-agarose binding studies allowed positive identification of this MBP kinase as p34cdc2.
Alvarez,
Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase.
1991, Pubmed
Alvarez,
Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase.
1991,
Pubmed Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed Behra,
Independent quantitation of the mitochondrial and the cytosolic isoenzyme of aspartate aminotransferase in chicken tissues by radioimmunoassays.
1981,
Pubmed Blenis,
Growth-regulated signal transduction by the MAP kinases and RSKs.
1991,
Pubmed Boulton,
Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies.
1991,
Pubmed Boulton,
ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.
1991,
Pubmed Boulton,
Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.
1991,
Pubmed Boulton,
An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control.
1990,
Pubmed Casillas,
Stimulation of B-cells via the membrane immunoglobulin receptor or with phorbol myristate 13-acetate induces tyrosine phosphorylation and activation of a 42-kDa microtubule-associated protein-2 kinase.
1991,
Pubmed Chen,
Nuclear localization and regulation of erk- and rsk-encoded protein kinases.
1992,
Pubmed Chung,
Coordinate regulation of pp90rsk and a distinct protein-serine/threonine kinase activity that phosphorylates recombinant pp90rsk in vitro.
1991,
Pubmed Chung,
Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity.
1991,
Pubmed
,
Echinobase Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed Clark-Lewis,
Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis-activated myelin basic protein kinase.
1991,
Pubmed
,
Echinobase Cobb,
Extracellular signal-regulated kinases: ERKs in progress.
1991,
Pubmed Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed Ettehadieh,
Tyrosyl phosphorylation and activation of MAP kinases by p56lck.
1992,
Pubmed
,
Echinobase Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed Gómez,
Dissection of the protein kinase cascade by which nerve growth factor activates MAP kinases.
1991,
Pubmed Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed Her,
Sequence of pp42/MAP kinase, a serine/threonine kinase regulated by tyrosine phosphorylation.
1991,
Pubmed Hoshi,
Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.
1988,
Pubmed Krek,
Casein kinase II is a predominantly nuclear enzyme.
1992,
Pubmed Krek,
Structure and developmental expression of the chicken CDC2 kinase.
1989,
Pubmed Kyriakis,
pp54 microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity.
1991,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Langlois,
Isolation of a transplantable cell line induced by the MC29 avian leukosis virus.
1974,
Pubmed Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
1987,
Pubmed Matsuda,
Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.
1992,
Pubmed Nakagawa,
A somatic cell-derived system for studying both early and late mitotic events in vitro.
1989,
Pubmed Nel,
CD-3-mediated activation of MAP-2 kinase can be modified by ligation of the CD4 receptor. Evidence for tyrosine phosphorylation during activation of this kinase.
1990,
Pubmed Nigg,
Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes.
1985,
Pubmed Northwood,
Isolation and characterization of two growth factor-stimulated protein kinases that phosphorylate the epidermal growth factor receptor at threonine 669.
1991,
Pubmed Okuda,
Granulocyte-macrophage colony-stimulating factor, interleukin-3, and steel factor induce rapid tyrosine phosphorylation of p42 and p44 MAP kinase.
1992,
Pubmed Pelech,
Mitogen-activated protein kinases: versatile transducers for cell signaling.
1992,
Pubmed Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed
,
Echinobase Peter,
Mitogen-activated protein kinases phosphorylate nuclear lamins and display sequence specificity overlapping that of mitotic protein kinase p34cdc2.
1992,
Pubmed Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Echinobase Pulverer,
Phosphorylation of c-jun mediated by MAP kinases.
1991,
Pubmed Ray,
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
1987,
Pubmed Ray,
Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
1988,
Pubmed Riabowol,
The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.
1989,
Pubmed Rossomando,
Biochemical characterization of a family of serine/threonine protein kinases regulated by tyrosine and serine/threonine phosphorylations.
1991,
Pubmed
,
Echinobase Sanghera,
Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.
1991,
Pubmed
,
Echinobase Sanghera,
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
1990,
Pubmed
,
Echinobase Sanghera,
Role of protein phosphorylation in the maturation-induced activation of a myelin basic protein kinase from sea star oocytes.
1991,
Pubmed
,
Echinobase Seger,
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.
1991,
Pubmed Stratton,
Electroconvulsive treatment induces a rapid and transient increase in tyrosine phosphorylation of a 40-kilodalton protein associated with microtubule-associated protein 2 kinase activity.
1991,
Pubmed Sturgill,
Recent progress in characterization of protein kinase cascades for phosphorylation of ribosomal protein S6.
1991,
Pubmed Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
