Dr. Julia Morales
Unfertilized sea urchin eggs are haploid cells arrested after completion of their meiotic divisions at the G1 stage of the cell cycle. Translation activity is mainly repressed in unfertilized eggs, and increases after fertilization, independently of mRNA transcription and ribosome biogenesis. De novo protein synthesis is required for cell cycle progression. All known factors involved in translation are present. Several translational initiation and elongation factors are activated at fertilization, and are involved in the protein synthesis increase. For example amongst the prominent results, the translation of cyclin B, a key regulator of cell cycle progression, was shown to be highly dependent on the availability of the eIF4E translation factor, and on the mTOR pathway. We also demonstrated a new role of the MAP kinase pathway implicated in an early step of chromatin/microtubule attachment during mitosis progression after fertilization. Furthermore, our data revealed the existence of a new cell cycle checkpoint involving the translational inhibitor 4E-BP. De novo protein synthesis triggered by fertilization is dependent on stored maternal mRNAs. We have developed polysome profiling in sea urchin embryo (6), to identify cellular mRNA(s) whose translational status is (are) modified following fertilization. We showed that only a restricted subset of maternal mRNAs is translated after fertilization, encoding proteins involved in regulatory pathways; moreover, the mTOR pathway plays a differential role in the translation of specific mRNAs. These data suggest for the first time the existence of an alternative to the cap-dependent translation in response to fertilization, and that this alternative translation could be involved in proper progression of the cell cycle after fertilization. We develop functional, structural and system biology approaches to study translational control at fertilization at both translational level (the translational machinery) and post‐translational level (signaling kinases).
Web Page: http://www.sb-roscoff.fr/en/team-translation-cell-cycle-and-development/research
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