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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396952
gRNA Name: LOC588806 gRNA28
Synonyms: Pks1#1sgRNA
Genomic Target: LOC588806
Derived from:

PAM Sequence: GGG
Sequence: 5' GGGGTCGATGTGAGGGTTTG 3'



Genomic Target S. purpuratus
Identities 18/20
genome
gRNA
 
5' CAAACCCCCACATCGACCCT 3'
   ||||||| ||||||||||| 
3' GTTTGGGAGTGTAGCTGGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716803..2716822 18/20 Antisense target LOC588806
NW_022145597.1:685643..685662 18/20 Antisense unknown
NW_022145606.1:29576035..29576054 18/20 Antisense unknown
NW_022145606.1:30900081..30900100 18/20 Antisense unknown
NW_022145594.1:51489815..51489834 17/20 Sense unknown
P. miniata 3.0 NW_024037069.1:4848293..4848312 19/20 Antisense off-target iars1
NW_024037047.1:14324443..14324462 18/20 Sense off-target LOC119730559
NW_024037057.1:3473755..3473774 18/20 Antisense off-target LOC119726019
NW_024037059.1:1512830..1512849 18/20 Sense off-target LOC119728883
NW_024037071.1:1733482..1733501 18/20 Sense unknown
L. variegatus 3.0 chr8:13270868..13270887 18/20 Antisense off-target LOC121420006
chr18:19090764..19090783 17/20 Antisense off-target LOC121405607
chr1:61934217..61934236 16/20 Antisense off-target LOC121431384
chr1:67528393..67528412 16/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Establishment of homozygous knock-out sea urchins., Curr Biol 2020  
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