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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396951
gRNA Name: gcml gRNA7
Synonyms: Hp GCM 351-374
Genomic Target: gcml
Derived from:

Sequence: 5' GGGCAGGAGAAGGGAACTAG 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' CTAGTTCCCTTCTCCTGCCA 3'
   ||||||||||||||||||| 
3' GATCAAGGGAAGAGGACGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145598.1:34773869..34773888 19/20 Sense target gcml
NW_022145570.1:4354743..4354762 16/20 Sense off-target LOC115929990
NW_022145594.1:39968715..39968734 16/20 Sense off-target LOC100889594
P. miniata 3.0 NW_024037068.1:15279254..15279273 17/20 Sense off-target LOC119736101
NW_024037075.1:6740134..6740153 16/20 Sense off-target ccdc47
NW_024037062.1:8371437..8371456 16/20 Antisense off-target LOC119731190
NW_024037074.1:26052648..26052667 18/20 Sense unknown
NW_024037051.1:17314902..17314921 18/20 Sense unknown
NW_024037059.1:36043260..36043279 18/20 Sense unknown
L. variegatus 3.0 chr11:7704753..7704772 16/20 Sense off-target LOC121424513
chr10:11552485..11552504 18/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Genetic manipulation of the pigment pathway in a sea urchin reveals distinct lineage commitment prior to metamorphosis in the bilateral to radial body plan transition., Sci Rep 2020     
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