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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396949
gRNA Name: gcml gRNA5
Synonyms: Hp GCM 263-286
Genomic Target: gcml
Derived from:

Sequence: 5' GGACGAGGTCGAGAGTTTGG 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' CCAAACTCTCGACCTCGTGC 3'
   |||||||||||||||||| |
3' GGTTTGAGAGCTGGAGCAGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145598.1:34773781..34773800 19/20 Sense target gcml
NW_022145609.1:4245994..4246013 16/20 Antisense off-target LOC105446740
NW_022145609.1:3597850..3597869 16/20 Antisense off-target LOC115928859
NW_022145609.1:3637243..3637262 16/20 Antisense off-target LOC115928830
NW_022145598.1:18467759..18467778 17/20 Antisense unknown
NW_022145612.1:38509314..38509333 16/20 Sense unknown
NW_022145602.1:8297493..8297512 16/20 Sense unknown
NW_022145609.1:3809424..3809443 16/20 Antisense unknown
P. miniata 3.0 NW_024037050.1:1943960..1943979 16/20 Antisense off-target LOC119744960
NW_024037059.1:20895123..20895142 17/20 Antisense unknown
L. variegatus 3.0 XR_005970524.1:804..823 16/20 off-target LOC121418888
XR_005970525.1:241..260 16/20 off-target LOC121418891
chr7:27304979..27304998 16/20 Antisense off-target LOC121418888
chr7:27720056..27720075 16/20 Antisense off-target LOC121418891
chr18:22600083..22600102 16/20 Antisense unknown
chr5:13763457..13763476 16/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Genetic manipulation of the pigment pathway in a sea urchin reveals distinct lineage commitment prior to metamorphosis in the bilateral to radial body plan transition., Sci Rep 2020     
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