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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396942
gRNA Name: LOC588806 gRNA22
Synonyms: HpPks1 sgRNA#2
Genomic Target: LOC588806
Derived from:

Sequence: 5' ACTGAGGGGTTTGAGGACAA 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' TTGTCCTCAAACCCCTCAGC 3'
   ||||||||||||||||||| 
3' AACAGGAGTTTGGGGAGTCA 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716401..2716420 19/20 Antisense target LOC588806
NW_022145612.1:15987739..15987758 17/20 Antisense off-target LOC105439527
P. miniata 3.0 NW_024037058.1:2801085..2801104 17/20 Sense off-target drg1
XR_005271529.1:1191..1210 16/20 off-target LOC119732247
XR_005271530.1:536..555 16/20 off-target LOC119732247
XR_005271531.1:503..522 16/20 off-target LOC119732247
XR_005271532.1:353..372 16/20 off-target LOC119732247
XR_005271533.1:354..373 16/20 off-target LOC119732247
XR_005271534.1:392..411 16/20 off-target LOC119732247
XR_005271535.1:1145..1164 16/20 off-target LOC119732247
XR_005271536.1:912..931 16/20 off-target LOC119732247
XR_005271537.1:455..474 16/20 off-target LOC119732247
XR_005271538.1:309..328 16/20 off-target LOC119732247
XR_005271539.1:904..923 16/20 off-target LOC119732247
XR_005271540.1:321..340 16/20 off-target LOC119732247
XR_005271541.1:474..493 16/20 off-target LOC119732247
XR_005271542.1:455..474 16/20 off-target LOC119732247
XR_005271543.1:469..488 16/20 off-target LOC119732247
XR_005271544.1:307..326 16/20 off-target LOC119732247
XR_005271545.1:345..364 16/20 off-target LOC119732247
NW_024037053.1:568267..568286 16/20 Sense off-target LOC119722309
NW_024037052.1:12976597..12976616 16/20 Antisense unknown
L. variegatus 3.0 chr6:37346923..37346942 18/20 Sense off-target LOC121417423
chr6:39560989..39561008 18/20 Sense off-target LOC121417465
chr7:7334652..7334671 18/20 Antisense off-target LOC121418329
chr15:27085113..27085132 17/20 Antisense off-target LOC121428528
chr2:17980091..17980110 17/20 Antisense unknown
chr6:15915435..15915454 17/20 Antisense unknown
chr6:16890444..16890463 17/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Establishment of knockout adult sea urchins by using a CRISPR-Cas9 system., Dev Growth Differ 2019  
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