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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396939
gRNA Name: LOC586240 gRNA2
Synonyms: HpFMO3 26-49
Genomic Target: LOC586240
Derived from:

Sequence: 5' GGGAGCTGGTGTGAGTGGCT 3'



Genomic Target S. purpuratus
Identities 18/20
genome
gRNA
 
5' AGCCACTCACACCAGCCCCT 3'
   |||||||||||||||| || 
3' TCGGTGAGTGTGGTCGAGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:35288213..35288232 18/20 Sense target LOC586240
P. miniata 3.0 NW_024037047.1:17113817..17113836 18/20 Sense off-target LOC119723935
NW_024037076.1:25177613..25177632 17/20 Antisense off-target LOC119720976
NW_024037076.1:25177761..25177780 16/20 Antisense off-target LOC119720976
NW_024037074.1:35720471..35720490 17/20 Antisense unknown
L. variegatus 3.0 chr9:33242099..33242118 18/20 Antisense off-target LOC121421789
chr13:7394136..7394155 16/20 Antisense off-target LOC121426614
chr13:32998314..32998333 17/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Genetic manipulation of the pigment pathway in a sea urchin reveals distinct lineage commitment prior to metamorphosis in the bilateral to radial body plan transition., Sci Rep 2020     
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