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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396938
gRNA Name: rhoa gRNA1
Synonyms: RhoA_sgRNA
Genomic Target: rhoa
Derived from:

PAM Sequence: TGG
Sequence: 5' AGGAAAAAGTTGGTTATCGT 3'



Genomic Target S. purpuratus
Identities 20/20
genome
gRNA
 
5' ACGATAACCAACTTTTTCCT 3'
   ||||||||||||||||||||
3' TGCTATTGGTTGAAAAAGGA 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145544.1:700700..700719 20/20 Antisense target rhoa
NW_022145612.1:45473451..45473470 20/20 Sense off-target LOC115919514
NW_022145612.1:45496813..45496832 19/20 Sense off-target LOC585526
NW_022145612.1:45555330..45555349 19/20 Sense off-target LOC585360
NW_022145612.1:45642208..45642227 19/20 Sense off-target LOC575855
NW_022145612.1:45522050..45522069 19/20 Sense off-target LOC115919286
NW_022145544.1:658094..658113 19/20 Antisense off-target LOC115917866
NW_022145544.1:680764..680783 19/20 Antisense off-target LOC115924486
NW_022145574.1:935195..935214 17/20 Sense off-target LOC590790
NW_022145526.1:2168256..2168275 17/20 Sense off-target LOC105444042
NW_022145604.1:9871492..9871511 16/20 Sense off-target LOC586484
NW_022145613.1:15509017..15509036 16/20 Sense off-target LOC578975
NW_022145613.1:21454746..21454765 16/20 Antisense off-target LOC585281
NW_022145602.1:15491764..15491783 16/20 Antisense off-target LOC591869
NW_022145612.1:45606436..45606455 19/20 Sense unknown
NW_022145594.1:34255177..34255196 18/20 Sense unknown
NW_022145594.1:34256276..34256295 18/20 Sense unknown
NW_022145594.1:34257371..34257390 18/20 Sense unknown
NW_022145594.1:34258476..34258495 18/20 Sense unknown
NW_022145594.1:34276665..34276684 18/20 Sense unknown
NW_022145526.1:1067636..1067655 17/20 Sense unknown
NW_022145605.1:22844698..22844717 16/20 Antisense unknown
NW_022145612.1:25405365..25405384 16/20 Sense unknown
NW_022145612.1:28558199..28558218 16/20 Antisense unknown
NW_022145566.1:2034560..2034579 16/20 Sense unknown
NW_022145598.1:14783232..14783251 16/20 Antisense unknown
P. miniata 3.0 NW_024037066.1:6189332..6189351 17/20 Sense off-target odr4
NW_024037072.1:11037748..11037767 17/20 Sense off-target LOC119741112
NW_024037064.1:800971..800990 16/20 Sense off-target LOC119733197
NW_024037064.1:690983..691002 16/20 Sense off-target LOC119733221
NW_024037074.1:15723975..15723994 16/20 Sense off-target LOC119746143
NW_024037057.1:15075657..15075676 17/20 Sense unknown
NW_024037064.1:1051443..1051462 16/20 Sense unknown
NW_024037064.1:309467..309486 16/20 Sense unknown
NW_024037064.1:322981..323000 16/20 Sense unknown
NW_024037064.1:628749..628768 16/20 Sense unknown
NW_024037064.1:819496..819515 16/20 Sense unknown
NW_024037073.1:2809782..2809801 16/20 Sense unknown
L. variegatus 3.0 chr13:31128653..31128672 17/20 Antisense off-target LOC121426009
chr16:2587779..2587798 17/20 Antisense off-target LOC121430175
chr16:2663884..2663903 17/20 Antisense off-target LOC121430166
chr3:65124742..65124761 17/20 Sense off-target LOC121411681
chr7:41779381..41779400 17/20 Antisense off-target LOC121419330
chr3:15403690..15403709 16/20 Antisense off-target kansl2
chr11:14164110..14164129 16/20 Sense off-target LOC121423672
chr3:48567540..48567559 16/20 Sense off-target LOC121411223
chr7:5008527..5008546 16/20 Sense off-target LOC121418266
chr9:4211513..4211532 16/20 Sense off-target LOC121421221
chr2:4691191..4691210 18/20 Sense unknown
chr5:44211760..44211779 17/20 Antisense unknown
chr5:45694244..45694263 17/20 Antisense unknown
chr9:24325451..24325470 17/20 Antisense unknown
chr1:83489989..83490008 16/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Partial exogastrulation due to apical-basal polarity of F-actin distribution disruption in sea urchin embryo by omeprazole., Genes Cells 2022     
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