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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396937
gRNA Name: bindin gRNA2
Synonyms: Hp bindin 325-348
Genomic Target: bindin
Derived from:

Sequence: 5' GGTTCAGTAACTGCCAGCAG 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' CTGCTGGCAGTTACTGAACG 3'
   ||||||||||||||||||| 
3' GACGACCGTCAATGACTTGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145604.1:6507630..6507649 19/20 Antisense target bindin
NW_022145602.1:10862492..10862511 18/20 Antisense off-target LOC581773
P. miniata 3.0 NW_024037066.1:2480379..2480398 17/20 Sense off-target LOC119734406
NW_024037075.1:8019955..8019974 17/20 Antisense off-target LOC119719236
NW_024037066.1:2394364..2394383 17/20 Sense off-target LOC119734560
NW_024037059.1:39406635..39406654 16/20 Antisense unknown
L. variegatus 3.0 chr11:34275235..34275254 16/20 Antisense off-target LOC121423598
Publications
???morpholino.displaySummary.papersFirst??? Bindin is essential for fertilization in the sea urchin., Proc Natl Acad Sci U S A 2021     
???morpholino.displaySummary.papersRecent??? Sperm lacking Bindin are infertile but are otherwise indistinguishable from wildtype sperm., Sci Rep 2021     
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