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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396936
gRNA Name: bindin gRNA1
Synonyms: Hp bindin 82-105
Genomic Target: bindin
Derived from:

Sequence: 5' GGGGCAGTCAGTAGGGGAGT 3'



Genomic Target S. purpuratus
Identities 20/20
genome
gRNA
 
5' ACTCCCCTACTGACTGCCCC 3'
   ||||||||||||||||||||
3' TGAGGGGATGACTGACGGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145604.1:6509478..6509497 20/20 Sense target bindin
NW_022145607.1:35025231..35025250 18/20 Antisense off-target LOC105440096
NW_022145605.1:24278816..24278835 17/20 Sense off-target LOC764716
NW_022145599.1:27728849..27728868 16/20 Sense off-target top3a
NW_022145609.1:36654671..36654690 16/20 Sense unknown
P. miniata 3.0 NW_024037056.1:13627850..13627869 19/20 Antisense off-target LOC119724707
NW_024037056.1:16790720..16790739 19/20 Sense off-target LOC119724921
NW_024037069.1:32964594..32964613 17/20 Antisense off-target LOC119737378
NW_024037073.1:21548143..21548162 17/20 Sense off-target LOC119742566
NW_024037074.1:21893199..21893218 17/20 Sense off-target LOC119744337
NW_024037076.1:3577491..3577510 17/20 Sense off-target LOC119720319
NW_024037059.1:22085335..22085354 17/20 Antisense off-target LOC119728740
NW_024037062.1:7665864..7665883 17/20 Antisense off-target LOC119731431
NW_024037063.1:2413009..2413028 16/20 Sense off-target bmp1l
NW_024037071.1:8238373..8238392 19/20 Sense unknown
NW_024037063.1:10441363..10441382 19/20 Sense unknown
NW_024037069.1:1189812..1189831 18/20 Sense unknown
NW_024037069.1:581650..581669 18/20 Antisense unknown
NW_024037069.1:605952..605971 18/20 Antisense unknown
NW_024037069.1:634101..634120 18/20 Sense unknown
NW_024037069.1:634185..634204 18/20 Sense unknown
NW_024037069.1:645970..645989 18/20 Sense unknown
NW_024037069.1:646090..646109 18/20 Sense unknown
NW_024037069.1:646194..646213 18/20 Sense unknown
NW_024037069.1:646298..646317 18/20 Sense unknown
NW_024037069.1:646406..646425 18/20 Sense unknown
NW_024037069.1:634153..634172 17/20 Sense unknown
NW_024037069.1:646058..646077 17/20 Sense unknown
NW_024037069.1:646162..646181 17/20 Sense unknown
NW_024037069.1:646266..646285 17/20 Sense unknown
NW_024037069.1:646374..646393 17/20 Sense unknown
L. variegatus 3.0 chr14:12250785..12250804 17/20 Sense off-target LOC121427203
chr4:31357076..31357095 17/20 Antisense unknown
chr4:39785137..39785156 17/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Bindin is essential for fertilization in the sea urchin., Proc Natl Acad Sci U S A 2021     
???morpholino.displaySummary.papersRecent??? Sperm lacking Bindin are infertile but are otherwise indistinguishable from wildtype sperm., Sci Rep 2021     
???morpholino.displaySummary.papersAll???