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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396864
gRNA Name: six1 gRNA2
Synonyms: Pm-Six1/2 86
Genomic Target: six1
Derived from:

Sequence: 5' CCGGCAGCGACCAGAGAAAA 3'



Genomic Target S. purpuratus P. miniata
Identities 18/20 20/20
genome
gRNA
 
5' TTTTCTCTGGTCGCTACCTG 3'
   ||||||||||||||| || |
3' AAAAGAGACCAGCGACGGCC 5'
 
5' TTTTCTCTGGTCGCTGCCGG 3'
   ||||||||||||||||||||
3' AAAAGAGACCAGCGACGGCC 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145611.1:28162822..28162841 18/20 Sense target six1
P. miniata 3.0 NW_024037051.1:5761138..5761157 20/20 Antisense target six1
NW_024037054.1:8497921..8497940 16/20 Sense off-target LOC119723231
NW_024037059.1:29526449..29526468 16/20 Sense off-target LOC119728541
L. variegatus 3.0 chr17:25009758..25009777 16/20 Sense off-target LOC121431402
Publications
???morpholino.displaySummary.papersFirst??? Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ., Nat Commun 2023     
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