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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395098
gRNA Name: LOC588806 gRNA19
Synonyms: Pks-sgRNA16
Genomic Target: LOC588806
Derived from:

Sequence: 5' GACAGCTGTTGCCAAACAG 3'



Genomic Target S. purpuratus
Identities 19/19
genome
gRNA
 
5' CTGTTTGGCAACAGCTGTC 3'
   |||||||||||||||||||
3' GACAAACCGTTGTCGACAG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2717065..2717083 19/19 Sense target LOC588806
NW_022145576.1:2547328..2547346 18/19 Sense off-target LOC764228
NW_022145604.1:20051588..20051606 15/19 Sense off-target LOC582047
NW_022145607.1:9369992..9370010 15/19 Antisense off-target LOC592380
NW_022145610.1:7319678..7319696 15/19 Antisense off-target LOC762398
NW_022145558.1:2369499..2369517 15/19 Sense off-target LOC105442597
NW_022145558.1:1168425..1168443 15/19 Antisense unknown
NW_022145558.1:1517319..1517337 15/19 Antisense unknown
NW_022145604.1:26397382..26397400 15/19 Sense unknown
P. miniata 3.0 NW_024037074.1:42179068..42179086 18/19 Sense off-target LOC119744418
NW_024037074.1:4150027..4150045 17/19 Antisense off-target LOC119744755
NW_024037057.1:10136050..10136068 17/19 Antisense off-target LOC119725742
NW_024037057.1:10136050..10136068 17/19 Antisense off-target LOC119725746
NW_024037050.1:4384606..4384624 16/19 Antisense off-target tat
NW_024037059.1:725135..725153 16/19 Antisense off-target LOC119728756
NW_024037068.1:8362633..8362651 16/19 Sense off-target LOC119735946
NW_024037055.1:14830492..14830510 16/19 Sense off-target LOC119723937
NW_024037056.1:2981364..2981382 16/19 Sense off-target LOC119724215
NW_024037056.1:5637083..5637101 16/19 Sense off-target LOC119724391
NW_024037059.1:19275487..19275505 15/19 Sense off-target LOC119727754
NW_024037069.1:16157114..16157132 15/19 Sense off-target LOC119737332
NW_024037069.1:16182614..16182632 15/19 Sense off-target LOC119736503
NW_024037069.1:16226658..16226676 15/19 Sense off-target LOC119736505
NW_024037069.1:16282372..16282390 15/19 Sense off-target LOC119736506
NW_024037073.1:14739516..14739534 15/19 Antisense off-target LOC119743285
NW_024037075.1:1476425..1476443 15/19 Sense off-target LOC119719444
NW_024037059.1:38907285..38907303 16/19 Antisense unknown
NW_024037069.1:40347114..40347132 16/19 Antisense unknown
NW_024037073.1:7192453..7192471 16/19 Sense unknown
NW_024037073.1:7331657..7331675 16/19 Sense unknown
NW_024037075.1:11261330..11261348 16/19 Sense unknown
NW_024037050.1:6692838..6692856 16/19 Antisense unknown
NW_024037056.1:1029948..1029966 16/19 Sense unknown
NW_024037069.1:16331383..16331401 15/19 Sense unknown
NW_024037069.1:46600786..46600804 15/19 Antisense unknown
NW_024037069.1:46641760..46641778 15/19 Antisense unknown
NW_024037058.1:8606583..8606601 15/19 Sense unknown
L. variegatus 3.0 chr1:22378467..22378485 17/19 Antisense off-target LOC121409406
chr15:27085777..27085795 17/19 Sense off-target LOC121428528
chr12:13444576..13444594 15/19 Antisense off-target LOC121425675
chr13:13768220..13768238 15/19 Sense off-target LOC121426959
chr2:25254279..25254297 15/19 Antisense off-target LOC121407672
chr8:34850398..34850416 15/19 Sense off-target LOC121419675
chr18:21503015..21503033 18/19 Antisense unknown
chr6:5073277..5073295 16/19 Antisense unknown
chr13:30797517..30797535 15/19 Sense unknown
chr3:60988279..60988297 15/19 Sense unknown
chr6:33225711..33225729 15/19 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
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