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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395096
gRNA Name: LOC588806 gRNA17
Synonyms: Pks-sgRNA14
Genomic Target: LOC588806
Derived from:

Sequence: 5' AGGAGCTGACTCCAGAAC 3'



Genomic Target S. purpuratus
Identities 18/18
genome
gRNA
 
5' GTTCTGGAGTCAGCTCCT 3'
   ||||||||||||||||||
3' CAAGACCTCAGTCGAGGA 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716962..2716979 18/18 Sense target LOC588806
NW_022145597.1:33411477..33411494 16/18 Antisense off-target LOC764645
NW_022145597.1:33013796..33013813 16/18 Antisense off-target LOC115918546
NW_022145551.1:1061269..1061286 15/18 Sense off-target LOC576603
NW_022145599.1:11770404..11770421 16/18 Sense unknown
P. miniata 3.0 NW_024037074.1:10511332..10511349 16/18 Sense off-target LOC119745274
NW_024037057.1:8011745..8011762 15/18 Sense off-target LOC119725626
NW_024037075.1:3584818..3584835 15/18 Antisense off-target LOC119719532
NW_024037075.1:3761729..3761746 15/18 Sense off-target LOC119719366
NW_024037047.1:14677844..14677861 17/18 Sense unknown
L. variegatus 3.0 chr4:24855451..24855468 16/18 Antisense off-target LOC121413674
chr12:19783400..19783417 15/18 Antisense off-target LOC121425181
chr4:22249119..22249136 16/18 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
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