Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase

Summary Attributions Wiki
ECB-GRNA-26395094
gRNA Name: alx1 gRNA1
Synonyms: Alx1-sgRNA1
Genomic Target: alx1
Derived from:

Sequence: 5' AGAACAGGCGCGCCAAGTGG 3'



Genomic Target S. purpuratus P. miniata
Identities 20/20 18/20
genome
gRNA
 
5' CCACTTGGCGCGCCTGTTCT 3'
   ||||||||||||||||||||
3' GGTGAACCGCGCGGACAAGA 5'
 
5' CCACTTGGCGCGCCGATTCT 3'
   ||||||||||||||  ||||
3' GGTGAACCGCGCGGACAAGA 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145603.1:14663157..14663176 20/20 Antisense target alx1
NW_022145612.1:10760171..10760190 18/20 Sense off-target unpgl
NW_022145599.1:35443334..35443353 16/20 Sense off-target LOC115923774
NW_022145607.1:11290514..11290533 18/20 Antisense unknown
P. miniata 3.0 NW_024037047.1:27178285..27178304 18/20 Sense target alx1
NW_024037062.1:710072..710091 18/20 Sense off-target LOC119731314
NW_024037059.1:41732444..41732463 17/20 Sense off-target otpl
NW_024037063.1:7004052..7004071 16/20 Antisense off-target LOC119732396
NW_024037047.1:971603..971622 18/20 Sense unknown
NW_024037048.1:10745276..10745295 18/20 Antisense unknown
NW_024037063.1:12551462..12551481 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
???morpholino.displaySummary.papersRecent???
???morpholino.displaySummary.papersAll???