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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395091
gRNA Name: LOC588806 gRNA14
Synonyms: Pks-sgRNA11
Genomic Target: LOC588806
Derived from:

Sequence: 5' GCAACTTTGGACATACCG 3'



Genomic Target S. purpuratus
Identities 18/18
genome
gRNA
 
5' CGGTATGTCCAAAGTTGC 3'
   ||||||||||||||||||
3' GCCATACAGGTTTCAACG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716698..2716715 18/18 Sense target LOC588806
NW_022145596.1:24624174..24624191 15/18 Sense off-target LOC583716
NW_022145615.1:25716353..25716370 15/18 Antisense off-target LOC576885
P. miniata 3.0 NW_024037048.1:9131436..9131453 16/18 Sense off-target LOC119737264
NW_024037064.1:4546481..4546498 15/18 Sense unknown
NW_024037068.1:23646994..23647011 15/18 Sense unknown
L. variegatus 3.0 chr15:27085410..27085427 18/18 Sense off-target LOC121428528
chr11:7877227..7877244 16/18 Antisense off-target LOC121423829
chr3:40643197..40643214 15/18 Antisense off-target LOC121411018
chr7:32261862..32261879 15/18 Antisense off-target LOC121419035
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
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