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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395088
gRNA Name: LOC588806 gRNA12
Synonyms: Pks-sgRNA9
Genomic Target: LOC588806
Derived from:

Sequence: 5' GGTGATCCTCTGGAAGCAG 3'



Genomic Target S. purpuratus
Identities 19/19
genome
gRNA
 
5' CTGCTTCCAGAGGATCACC 3'
   |||||||||||||||||||
3' GACGAAGGTCTCCTAGTGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716616..2716634 19/19 Sense target LOC588806
NW_022145607.1:34977588..34977606 17/19 Sense off-target LOC591886
NW_022145603.1:21871575..21871593 16/19 Antisense off-target LOC115925494
NW_022145601.1:14017937..14017955 16/19 Sense off-target LOC115924519
NW_022145615.1:10277723..10277741 15/19 Antisense off-target herc1
NW_022145594.1:16364442..16364460 16/19 Antisense unknown
P. miniata 3.0 NW_024037047.1:22661156..22661174 18/19 Antisense off-target LOC119725759
NW_024037061.1:6146373..6146391 15/19 Sense off-target otc
NW_024037059.1:7334124..7334142 15/19 Sense off-target LOC119728774
NW_024037069.1:20726427..20726445 15/19 Sense unknown
L. variegatus 3.0 chr15:27085328..27085346 18/19 Sense off-target LOC121428528
chr5:45272835..45272853 17/19 Antisense off-target LOC121416273
chr5:46631337..46631355 17/19 Antisense off-target LOC121416303
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
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